Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China.
Biol Trace Elem Res. 2011 Dec;144(1-3):647-56. doi: 10.1007/s12011-011-9018-9. Epub 2011 Mar 16.
The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on intestinal immune function by histopathological observation of cecal tonsil and changes of the cecal tonsil T cell subsets by method of flow cytometry. Four hundred twenty 1-day-old avian broilers were divided into six groups and fed on a corn-soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, and 60 mg/kg vanadium supplied as ammonium metavanadate. In comparison with those of control group, lymphocytes in the lymphatic nodule of cecal tonsil were apparently decreased in 45 and 60 mg/kg groups. The percentage of CD(3)(+) T cells was decreased (p < 0.05) in 45 mg/kg group at 28 and 42 days of age and significantly decreased (p < 0.01) in 60 mg/kg group at 28 and 42 days of age. The percentages of CD(3)(+)CD(4)(+) and CD(3)(+)CD(8)(+) T cells were markedly decreased (p < 0.05 or p < 0.01) in 60 mg/kg group from 14 to 28 days of age and were decreased (p < 0.05) in 45 mg/kg group at 28 and 42 days of age. However, changes of the CD(4)(+)/CD(8)(+) ratio were not significant. Meanwhile, the cecal tonsil interleukin-2 (IL-2) contents were decreased (p < 0.05 or p < 0.01) in 45 and 60 mg/kg groups from 14 to 42 days of age. It was concluded that dietary vanadium in excess of 30 mg/kg reduced the percentages of cecal tonsil T cells subsets and IL-2 contents, and caused cecal tonsil lesions, which impaired cecal tonsil function and impacted the local mucosal immune function of the intestines in broilers.
本 42 天研究旨在通过对盲肠扁桃体的组织病理学观察以及采用流式细胞术检测盲肠扁桃体 T 细胞亚群的变化来研究膳食过量钒对肠道免疫功能的影响。将 420 只 1 日龄禽肉鸡分为 6 组，分别用玉米-豆粕基础日粮（对照组）或相同日粮添加 5、15、30、45 和 60mg/kg 偏钒酸铵作为钒源进行饲养。与对照组相比，45 和 60mg/kg 组盲肠扁桃体淋巴小结中的淋巴细胞明显减少。28 和 42 日龄时，45mg/kg 组 CD(3)(+)T 细胞的百分比降低（p<0.05），28 和 42 日龄时，60mg/kg 组 CD(3)(+)T 细胞的百分比显著降低（p<0.01）。14-28 日龄时，60mg/kg 组 CD(3)(+)CD(4)(+)和 CD(3)(+)CD(8)(+)T 细胞的百分比明显降低（p<0.05 或 p<0.01），28 和 42 日龄时，45mg/kg 组 CD(3)(+)CD(4)(+)和 CD(3)(+)CD(8)(+)T 细胞的百分比降低（p<0.05）。然而，CD(4)(+)/CD(8)(+)的比值变化不显著。同时，14-42 日龄时，45 和 60mg/kg 组盲肠扁桃体白细胞介素-2（IL-2）含量降低（p<0.05 或 p<0.01）。结果表明，日粮中添加超过 30mg/kg 的钒会降低盲肠扁桃体 T 细胞亚群和 IL-2 的含量，并导致盲肠扁桃体损伤，从而损害盲肠扁桃体的功能，影响肉鸡肠道局部黏膜免疫功能。
