Department of Oncology, General Hospital, Jinan Command of the People's Liberation Army, 250031 Jinan, China.
Med Oncol. 2011 Dec;28 Suppl 1:S577-85. doi: 10.1007/s12032-010-9734-6. Epub 2010 Nov 3.
HuR is a ubiquitously expressed RNA-binding protein that stabilizes the mRNAs of certain genes and regulates the translation to proteins. Elevated cytoplasmic expression of HuR has been suggested to be associated with reduced survival in a wide variety of human carcinomas. However, the clinical significance of HuR expression in lung cancer remains unknown. In this study, we examined HuR expression in 132 patients with non-small cell lung carcinoma (NSCLC) by means of immunohistochemistry and correlated clinicopathologic data, lymphatic microvessel density (LVD), or microvessel density (MVD) with HuR immunostaining. HuR was expressed in 80.3% (106/132) of cases and was predominantly localized in the nucleus. Cytoplasmic HuR expression occurred in 40.9% (54/132) of NSCLC specimens and was associated with high MVD and LVD. In univariate analysis, cytoplasmic HuR, but not nuclear HuR expression was found to significantly influence the relapse-free survival and overall survival. In addition, cytoplasmic expression of HuR was identified as an independent prognostic factor for survival in multivariate analysis. Our data provide evidence for a clinically prognostic role of HuR in NSCLC and demonstrate an association between HuR expression, and angiogenesis and lymphangiogenesis.
HuR 是一种广泛表达的 RNA 结合蛋白，可稳定某些基因的 mRNA 并调节翻译为蛋白质。细胞质中 HuR 的表达升高与多种人类癌的存活率降低有关。然而，HuR 表达在肺癌中的临床意义尚不清楚。在这项研究中，我们通过免疫组织化学检测了 132 例非小细胞肺癌 (NSCLC) 患者的 HuR 表达，并将临床病理数据、淋巴管微脉管密度 (LVD) 或微血管密度 (MVD) 与 HuR 免疫染色相关联。HuR 在 80.3%（106/132）的病例中表达，主要定位于细胞核。细胞质 HuR 表达发生在 40.9%（54/132）的 NSCLC 标本中，与高 MVD 和 LVD 相关。在单因素分析中，细胞质 HuR 而非核 HuR 表达被发现显著影响无复发生存和总生存。此外，细胞质 HuR 的表达被确定为多因素分析中生存的独立预后因素。我们的数据为 HuR 在 NSCLC 中的临床预后作用提供了证据，并表明 HuR 表达与血管生成和淋巴管生成之间存在关联。
