Hu Peng, Ni Chengyao, Teng Pen
Department of Cardiac and Great Vessel Surgery, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
Bioengineered. 2022 Mar;13(3):6590-6599. doi: 10.1080/21655979.2022.2042141.
We aimed to assess the effects of artesunate (ART) on the proliferation, migration, invasion and apoptosis of the non-small cell lung cancer cells A549 and H1299. The effects of ART and carboplatin (CBP) alone or in combination on the viability of A549 and H1299 cells were evaluated by MTT assay. The effects of 30 μg/ml ART on cell invasion, migration and apoptosis were evaluated by Transwell assay, scratch assay and flow cytometry, respectively. The protein expressions of human antigen R (HuR) and MMP-9 after treatment with 30 μg/ml ART for 48 h were detected by Western blotting. After 48 h of treatment, 9 μg/ml ART in combination with 7 μg/ml CBP exerted a mild synergistic effect on cell viability. The migration rates of cells treated with 30 μg/ml ART and number of invasive cells were significantly lower, and the apoptosis rates were higher than those of the DMSO-treated group. HuR and MMP-9 expressions in cells treated with 30 μg/ml ART for 48 h were significantly lower than those of the DMSO-treated group. ART suppresses the proliferation, migration and invasion of A549 and H1299 cells and induces their apoptosis, probably being associated with decreased expressions of HuR and MMP-9 proteins.
我们旨在评估青蒿琥酯（ART）对非小细胞肺癌细胞A549和H1299增殖、迁移、侵袭及凋亡的影响。通过MTT法评估ART和卡铂（CBP）单独或联合使用对A549和H1299细胞活力的影响。分别通过Transwell法、划痕法和流式细胞术评估30μg/ml ART对细胞侵袭、迁移及凋亡的影响。通过蛋白质印迹法检测30μg/ml ART处理48小时后人类抗原R（HuR）和基质金属蛋白酶-9（MMP-9）的蛋白表达。处理48小时后，9μg/ml ART与7μg/ml CBP联合使用对细胞活力产生轻度协同作用。30μg/ml ART处理的细胞迁移率和侵袭细胞数量显著降低，凋亡率高于二甲基亚砜（DMSO）处理组。30μg/ml ART处理48小时的细胞中HuR和MMP-9表达显著低于DMSO处理组。ART抑制A549和H1299细胞的增殖、迁移和侵袭并诱导其凋亡，可能与HuR和MMP-9蛋白表达降低有关。
