School of Biological Sciences, Flinders University, Adelaide, SA 5001, Australia.
J Microbiol. 2010 Oct;48(5):644-56. doi: 10.1007/s12275-010-9370-z. Epub 2010 Nov 3.
Nucleotide excision repair (NER) rectifies a variety of chemically and structurally distinct DNA lesions. The current model of NER is based upon the enteric bacterium Escherichia coli and there is scarce information about how other bacterial species respond to, and correct, DNA damage. Here we report the isolation and functional analysis of the uvrA and uvrB genes from Vibrio natriegens, a naturally occurring marine bacterium. Genetic studies were completed to assess the repair capabilities of V. natriegens uvrA and uvrB in E. coli uvrA and uvrB mutants. In addition to the genetic studies, transcriptional fusions between the luciferase gene and the 5' regulatory regions of uvrA and uvrB gene of V. natriegens and E. coli were constructed. Luminescent measurements from E. coli transformed with these constructs showed that whilst the response to UV irradiation of either E. coli or V. natriegens uvrA regulatory sequences was similar, both the rate and induction of luminescence detected from the uvrB regulatory regions differed.
核苷酸切除修复 (NER) 纠正了多种化学和结构上不同的 DNA 损伤。目前的 NER 模型基于肠道细菌大肠杆菌，关于其他细菌如何应对和纠正 DNA 损伤的信息很少。在这里，我们报告了来自海洋细菌水生栖热菌的 uvrA 和 uvrB 基因的分离和功能分析。进行了遗传研究以评估 V. natriegens uvrA 和 uvrB 在大肠杆菌 uvrA 和 uvrB 突变体中的修复能力。除了遗传研究外，还构建了荧光素酶基因与 V. natriegens 和大肠杆菌 uvrA 和 uvrB 基因的 5'调控区之间的转录融合。用这些构建体转化的大肠杆菌的发光测量表明，尽管大肠杆菌或 V. natriegens uvrA 调控序列对紫外线照射的反应相似，但从 uvrB 调控区检测到的发光的速率和诱导都不同。
