The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education and The State Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing, 100871, China.
Endocrine. 2010 Oct;38(2):188-93. doi: 10.1007/s12020-010-9371-z. Epub 2010 Jul 11.
The focal adhesion-associated protein (FAAP), encoded by the murine D10Wsu52e gene, is named as involved in modulating cell adhesion dynamics. It is a highly conserved and ubiquitously expressed protein, and its human homologue HSPC117 has been identified in many protein complexes. However, the expression and regulation of the FAAP gene have not yet been well characterized. Herein, we demonstrate that FAAP mRNA and protein expression are highly regionalized in the mouse epididymis with predominant enrichment in the initial segment. During sexual maturation, FAAP mRNA is always expressed in the caput epididymides. Castration causes rapid and significant decrease of FAAP mRNA abundance within the initial segment, whereas testosterone replacement fails to reverse the regression. Unilateral orchidectomy and efferent duct ligation studies further validate that expression of the FAAP mRNA is highly dependent on the presence of luminal testicular factors rather than testosterone. Furthermore, FAAP expression in the initial segment is not affected by cryptorchism.
该黏着斑相关蛋白（FAAP）由鼠的 D10Wsu52e 基因编码，因其参与调节细胞黏附动力学而得名。它是一种高度保守且广泛表达的蛋白，其人类同源物 HSPC117 已在许多蛋白复合物中被鉴定。然而，FAAP 基因的表达和调控尚未得到很好的描述。本文作者证明，FAAP mRNA 和蛋白在小鼠附睾中具有高度区域性表达，在起始段富集。在性成熟过程中，FAAP mRNA 始终在附睾头部表达。去势导致初始段中 FAAP mRNA 丰度迅速且显著降低，而睾酮替代未能逆转这种退化。单侧睾丸切除术和输出管结扎研究进一步证实，FAAP mRNA 的表达高度依赖于管腔睾丸因子的存在，而不是睾酮。此外，隐睾症并不影响 FAAP 在起始段的表达。
