Zhao Jiangdong, Ju Xiaofang, Zhang Liqiang, An Yang, Zhang Zhenwu, Sun Zhongwu, Ding Naizheng, Teng Chun-Bo
Laboratory of Animal Development Biology, College of Life Science, Northeast Forestry University, Harbin, China.
Front Biosci (Elite Ed). 2011 Jan 1;3(1):355-63. doi: 10.2741/e250.
D10Wsu52e is a recently discovered and highly conserved mouse gene. FAAP, the protein encoded by D10Wsu52e, participates in regulation of integrin-based focal adhesions. To explore the function of FAAP in pancreas development, we assessed the spatiotemporal expression of D10Wsu52e, paxillin and vinculin in the developing mouse pancreas through quantitative RT-PCR, in situ hybridization and histochemistry methods. Our results show that, at e9.5 and e10.5, D10Wsu52e mRNA is mainly expressed in the brain, optic vesicles, otic vesicles, limb buds, somites and gut, and also in the pancreatic buds at e10.5. Subsequently, D10Wsu52e mRNA is expressed mainly in the epithelial progenitors at e12.5, then decreases in the endocrine precursors and ductal cells, whereas still maintains in the exocrine precursors until e15.5. At e17.5, D10Wsu52e mRNA is highly expressed in exocrine acinar cells, but weakly in ductal and endocrine cells. Furthermore, the expression patterns of paxillin and vinculin mRNAs are similar to D10Wsu52e from e12.5 to e15.5, which suggests that D10Wsu52e may be related to the acinar development, adhesion and migration of progenitors and endocrine precursors.
D10Wsu52e是最近发现的一个高度保守的小鼠基因。D10Wsu52e编码的蛋白质FAAP参与基于整合素的粘着斑的调节。为了探究FAAP在胰腺发育中的功能，我们通过定量逆转录聚合酶链反应、原位杂交和组织化学方法评估了D10Wsu52e、桩蛋白和纽蛋白在发育中小鼠胰腺中的时空表达。我们的结果表明，在胚胎第9.5天和第10.5天，D10Wsu52e mRNA主要在脑、视泡、听泡、肢芽、体节和肠道中表达，在胚胎第10.5天的胰腺芽中也有表达。随后，D10Wsu52e mRNA在胚胎第12.5天主要在上皮祖细胞中表达，然后在内分泌前体细胞和导管细胞中减少，而在外分泌前体细胞中一直维持到胚胎第15.5天。在胚胎第17.5天，D10Wsu52e mRNA在外分泌腺泡细胞中高表达，但在导管和内分泌细胞中低表达。此外，从胚胎第12.5天到第15.5天，桩蛋白和纽蛋白mRNA的表达模式与D10Wsu52e相似这表明D10Wsu52e可能与祖细胞和内分泌前体细胞的腺泡发育、粘附及迁移有关。
