Department of Obstetric & Gynecology, Trakya University Medical Faculty, Edirne, Turkey.
Neurourol Urodyn. 2011 Jan;30(1):126-32. doi: 10.1002/nau.20972.
The aim of this study was to compare neuromuscular histomorphometry of the uterine ligaments and vaginal wall in women with and without pelvic organ prolapse.
Biopsies were obtained from the round, uterosacral, and cardinal ligaments of the uterus and apical vaginal wall of women having pelvic organ prolapse repaired (stage ≥ II; prolapse group, 37) and the same location in patients with no prolapse (stage < II; control group, 47). Routine hematoxylin-eosin (H & E) staining and immunohistochemical staining for Protein Gene Product 9.5 (PGP 9.5) and smooth muscle α-actin were performed for all specimens.
Smooth muscle percentage of the uterosacral and cardinal ligaments were not significantly different in women with prolapse than in women without. In round ligament, mean smooth muscle percentage was lower than in women with normal support (81.63 ± 8.2 vs. 51.63 ± 16, P=0.000). Mean distance of the smooth muscle fibers from surface epithelium of the vaginal epithelium of the women with prolapse were significantly higher than the control group (1.679 ± 0.34 vs. 2.240 ± 0.33, P = 0.000). PGP 9.5 stained area percentage of uterine ligaments and vaginal wall tissue samples were significantly lower in women with prolapse.
Both total innervation of the anterior vaginal epithelium and uterine ligaments, and muscular percentage of the round ligament and vaginal wall were decreased in women with pelvic organ prolapse.
本研究旨在比较有和无盆腔器官脱垂（POP）女性的子宫韧带和阴道壁的神经肌肉组织形态计量学。
从行盆腔器官脱垂修复术（POP 分期≥II 期；脱垂组，37 例）的女性的子宫圆韧带、子宫骶骨韧带和主韧带以及阴道顶端活检，以及无脱垂（POP 分期<II 期；对照组，47 例）的女性的相同部位获取活检。对所有标本进行常规苏木精-伊红（H & E）染色和蛋白基因产物 9.5（PGP 9.5）和平滑肌α-肌动蛋白免疫组织化学染色。
与无脱垂的女性相比，脱垂女性的子宫骶骨韧带和主韧带的平滑肌百分比没有显著差异。在圆韧带中，平均平滑肌百分比低于正常支撑的女性（81.63 ± 8.2 对 51.63 ± 16，P=0.000）。脱垂女性阴道上皮表面的平滑肌纤维距离明显高于对照组（1.679 ± 0.34 对 2.240 ± 0.33，P=0.000）。子宫韧带和阴道壁组织样本的 PGP 9.5 染色面积百分比在脱垂女性中明显较低。
在患有盆腔器官脱垂的女性中，前阴道上皮和子宫韧带的总神经支配以及圆韧带和阴道壁的肌肉百分比均降低。
