Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning Province, PR China.
Phytochem Anal. 2011 May-Jun;22(3):230-5. doi: 10.1002/pca.1270. Epub 2010 Nov 2.
Cortex Mori, one of the well-known traditional Chinese herbal medicines, is derived from the root bark of Morus alba L. according to the China Pharmacopeia. Stilbene glycosides are the main components isolated from aqueous extracts of Morus alba and their content varies depending on where Cortex Mori was collected. We have established a qualitative and quantitative method based on the bioactive stilbene glycosides for control of the quality of Cortex Mori from different sources.
To develop a high-performance liquid chromatography coupled with ultraviolet absorption detection for simultaneous quantitative determination of five major characteristic stilbene glycosides in 34 samples of the root bark of Morus alba L. (Cortex Mori) from different sources.
The analysis was performed on an ODS column using methanol-water-acetic acid (18: 82: 0.1, v/v/v) as the mobile phase and the peaks were monitored at 320 nm.
All calibration curves showed good linearity (r ≥ 0.9991) within test ranges. This method showed good repeatability for the quantification of these five components in Cortex Mori with intra- and inter-day standard deviations less than 2.19% and 1.45%, respectively.
The validated method was successfully applied to quantify the five investigated components, including a pair of cis-trans-isomers 1 and 2 and a pair of isomers 4 and 5 in 34 samples of Cortex Mori from different sources.
桑白皮是一种著名的中药，来源于桑科植物桑的根皮，《中国药典》有记载。从桑白皮水提物中分离得到的主要成分为二苯乙烯苷类化合物，其含量因桑白皮的产地不同而有所差异。我们建立了一种基于生物活性二苯乙烯苷类化合物的定性定量方法，用于控制不同来源桑白皮的质量。
建立一种高效液相色谱法，同时测定 34 批不同产地桑白皮（桑白皮）中二苯乙烯苷类 5 种特征成分的含量。
采用 ODS 柱，以甲醇-水-冰醋酸（18：82：0.1，v/v/v）为流动相，在 320nm 处检测。
所有校准曲线均呈良好的线性（r≥0.9991）。该方法用于测定桑白皮中二苯乙烯苷类 5 种成分的含量，日内和日间精密度的标准偏差分别小于 2.19%和 1.45%。
该方法可用于定量测定 34 批不同产地桑白皮中二苯乙烯苷类 5 种成分，包括一对顺式-反式异构体 1 和 2 以及一对异构体 4 和 5。
