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[桑白皮中桑辛素的测定]

[Determination of morusin in cortex mori].

作者信息

Zong Yu-ying, Ip Siu-po, Dong Ting-xia, Che Chun-tao

机构信息

School of Chinese Medicine, The Chinese University of Hongkong, Shatin, Hongkong, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(11):1038-40.

Abstract

OBJECTIVE

To determine the contents of morusin in Sangbaipi (Cortex Mori) of different commercial grades and species and obtained from different regions.

METHOD

Contents of morusin were determined by HPLC on an Alltima C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water (58:42) as mobile phase. UV detection was conducted at 270 nm.

RESULT

The amounts of morusin were found to vary among samples of Cortex Mori bearing a yellow-brown outer layer and those without the yellow-brown cork layer. The morusin contents were also related to the thickness of Cortex Mori.

CONCLUSION

The method was simple, reproducible and reliable. It can be applied to the quality assessment of Cortex Mori.

摘要

目的

测定不同商品等级、品种及产地桑白皮中桑辛素的含量。

方法

采用高效液相色谱法,在Alltima C18柱(4.6 mm×250 mm,5μm)上,以乙腈-水(58:42)为流动相,于270 nm波长处进行紫外检测,测定桑辛素含量。

结果

发现带有黄棕色外层的桑白皮样品与无黄棕色栓皮层的样品中桑辛素含量不同。桑辛素含量还与桑白皮的厚度有关。

结论

该方法简便、重现性好且可靠,可用于桑白皮的质量评价。

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