Sun Xinguang, Huang Wenhua, Ma Miao, Guo Baolin, Wang Guiying
College of Life Science, Shihezi University, Shihezi 832003, China.
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2079-82.
To develop a HPLC method for the determination of arbutin, bergenin and catechin in Chinese herb Bergenia, and to provide a scientific basis for evaluating the quality and reasonable utilization of the herb.
The HPLC analysis was achieved by using a C18 column and methanol-water as the mobile phase, with a flow rate of 1.0 mL min(-1), and detected by UV at 275 nm. The contents of arbutin, bergenin and catechin in the different parts of axial root, fibrous root and blade from Bergenia purpurascens and B. crassifolia.
The contents of arbutin, bergenin and catechin have a few difference in B. purpurascens and B. crassifolia, and varies significantly in the different part of axial root, fibrous root and blade from some species. The contents of bergenin are higher in axial root, fibrous root, and the content of arbutin is higher in blade.
This HPLC method can be used to determine simultaneously the content of arbutin, bergenin and catechin, and can establish a foundation for scientific study and evaluating the quality of species in Bergenia.
建立高效液相色谱法测定中药岩白菜中熊果苷、岩白菜素和儿茶素的含量，为评价该药材质量及合理利用提供科学依据。
采用C18色谱柱，以甲醇 - 水为流动相，流速为1.0 mL·min⁻¹，于275 nm波长处采用紫外检测进行高效液相色谱分析，测定紫花岩白菜和厚叶岩白菜主根、须根及叶片不同部位中熊果苷、岩白菜素和儿茶素的含量。
紫花岩白菜和厚叶岩白菜中熊果苷、岩白菜素和儿茶素的含量存在一定差异，且某些品种的主根、须根及叶片不同部位间差异显著。岩白菜素在主根、须根中含量较高，熊果苷在叶片中含量较高。
该高效液相色谱法可同时测定熊果苷、岩白菜素和儿茶素的含量，为岩白菜属植物的科学研究及质量评价奠定基础。
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