采用高效薄层层析-二苯基苦味酰基自由基（HPTLC-DPPH*）法对厚叶岩白菜绿色、棕色和黑色叶片中酚类成分进行分离及自由基清除活性评价。

Separation and evaluation of free radical-scavenging activity of phenol components of green, brown, and black leaves of Bergenia crassifolia by using HPTLC-DPPH* method.

作者信息

Pozharitskaya Olga N, Ivanova Svetlana A, Shikov Alexander N, Makarov Valery G, Galambosi Bertalan

机构信息

Saint-Petersburg Institute of Pharmacy, St. Petersburg, Russia.

出版信息

J Sep Sci. 2007 Oct;30(15):2447-51. doi: 10.1002/jssc.200700178.

DOI:10.1002/jssc.200700178

PMID:17763526

Abstract

A new procedure has been developed to separate and quantify the free radical-scavenging activity of individual compounds from green, brown, and black leaves of Bergenia crassifolia based on the combination of high performance TLC (HPTLC) with a diode array detector (DAD) and postchromatographic 1,1-diphenyl-2-picrylhydrazyl radical (DPPH(*)) derivatization. Free gallic and ellagic acids, arbutin, hydroquinone, and bergenin in the B. crassifolia leaves' extracts were separated by HPTLC and identified. All compounds of the extract excluding bergenin were capable of scavenging DPPH * radicals. From the estimated ID(50) values, it can be seen that the increasing order of activity was gallic acid > arbutin > ellagic acid > hydroquinone > ascorbic acid. The antiradical activity of leaves of B. crassifolia is probably associated to the presence of phenol.

摘要

基于高效薄层色谱（HPTLC）与二极管阵列检测器（DAD）相结合以及色谱后1,1-二苯基-2-苦基肼自由基（DPPH*）衍生化，开发了一种新方法，用于分离和定量厚叶岩白菜绿色、棕色和黑色叶片中单个化合物的自由基清除活性。通过HPTLC分离并鉴定了厚叶岩白菜叶提取物中的游离没食子酸、鞣花酸、熊果苷、对苯二酚和岩白菜素。提取物中除岩白菜素外的所有化合物均能够清除DPPH*自由基。从估计的半数抑制浓度（ID50）值可以看出，活性增加顺序为没食子酸＞熊果苷＞鞣花酸＞对苯二酚＞抗坏血酸。厚叶岩白菜叶的抗自由基活性可能与酚类物质的存在有关。