连翘中连翘苷的含量测定
[Quantitative determination of forsythiaside in Forsythia suspensa].
作者信息
Xia Bohou, Zhu Jingjing, Wang Zhimin, Lin Limei, Gao Huimin
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2110-2.
OBJECTIVE
To develop a quantitative determination of Forsythia suspensa and to provide scientific basis for its quality control.
METHOD
By HPLC, the separation was performed on a Zorebax Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a isocratic elution of acetonitrile-water (containing 0.4% glacial acetic acid, 15:85). The detection wavelength was 330 nm.
RESULT
The calibration curve showed a good linearity (r = 0.9998) within test range of 0.202-1.515 microg. And the average recovery was 98.54% with RSD 1.1%. The content range of forsythoside A in 11 batches of Forsythiae Fructus was 0.200%-1.681%.
CONCLUSION
The developed method is simple, accurate, specific, and with good repeatability. It is suitable for quality evaluation of Forsythiae Fructus.
目的
建立连翘的含量测定方法，为其质量控制提供科学依据。
方法
采用高效液相色谱法，以Zorebax Eclipse XDB-C18柱（4.6 mm×250 mm，5μm）为分离柱，在25℃下，以乙腈-水（含0.4%冰醋酸，15:85）等度洗脱，检测波长为330 nm。
结果
在0.202 - 1.515μg的检测范围内，校准曲线线性关系良好（r = 0.9998）。平均回收率为98.54%，相对标准偏差为1.1%。11批连翘果实中连翘苷A的含量范围为0.200% - 1.681%。
结论
所建立的方法简便、准确、专属性强、重复性好，适用于连翘果实的质量评价。
Zotero 插件