[反相高效液相色谱法测定乌药中去甲异波尔定的含量]
[Determination of norisoboldine in Radix Lindera by RP-HPLC].
作者信息
Chen Jianzhong, Chou Guixin, Yang Li, Wang Changhong, Wang Zhengtao
机构信息
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(21):2774-6.
OBJECTIVE
To develop a RP-HPLC method for quantitative determination of norisoboldine in Radix Linderae and to provide valuable data for quality control of Radix Linderae.
METHOD
The separation was performed on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a gradient elution of mobile phase A (0.5% formic acid, adjusted pH 2.25 with triethylamine) and mobile phase B (acetonitrile). The detection wavelength was 280 nm.
RESULT
The calibration curve showed a good linearity (r = 0.999 9) within test ranges of 0.015-1.509 microg. The average recovery was 99.58% with RSD 1.4%.
CONCLUSION
The developed method is simple, accurate and reliable with good repeatability. It is suitable for quality evaluation of Radix Linderae.
目的
建立一种反相高效液相色谱法（RP-HPLC）用于定量测定乌药中的去甲异波尔定，并为乌药的质量控制提供有价值的数据。
方法
采用菲罗门Gemini C18柱（4.6 mm×250 mm，5μm），在25℃下进行分离，流动相A（0.5%甲酸，用三乙胺调pH至2.25）和流动相B（乙腈）进行梯度洗脱。检测波长为280 nm。
结果
在0.015 - 1.509μg的测试范围内，校准曲线呈现良好的线性关系（r = 0.999 9）。平均回收率为99.58%，相对标准偏差（RSD）为1.4%。
结论
所建立的方法简便、准确、可靠，重复性良好。适用于乌药的质量评价。
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