[软枣猕猴桃提取物抗肿瘤作用的实验研究]
[Experimental study on antitumor effects of extracts from Actinidia argutaor].
作者信息
Wang Lan, Kang Chen, Yang Weipeng, Li Manling, Wang Yanli, Wang Yiwei, Hang Luqi, Liang Rixin, Yang Qing, Yin Xiaojie
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2184-6.
OBJECTIVE
To observe the inhibitory effect of extract from Actinidia argutaor on in vivo and in vitro carcinomata, and explore its mechanism.
METHOD
The in vivo S180 and H22 model were used to observe the effect of A. argutaor on inhibitory rate of carcinomata, organ relative weight of spleen and thymus gland and the release of tumor necrosis factor. A549 cells were exposed to extract from A. argutaor with different concentrations for 24, 48, 72 hours. MTT assay was used to evaluate the inhibiting effects of extract from A. argutaor on the proliferation of the cells. Flow cytometry was applied to detect cell cycle.
RESULT
The inhibitory effects of the extracts on in vivo and in vitro carcinomata were observed, the inhibitory rate for S180 and H22 line was 33.32% and 34.62% respectively. The extracts could inhibit the proliferation of A549 cells during G0-G1 period and significantly decrease the cell ratio of S stage.
CONCLUSION
The extracts from A. argutaor showed a good antineoplastic activity.
目的
观察软枣猕猴桃提取物对体内外肿瘤的抑制作用，并探讨其作用机制。
方法
采用体内S180和H22模型，观察软枣猕猴桃对肿瘤抑制率、脾脏和胸腺脏器系数及肿瘤坏死因子释放的影响。将A549细胞分别用不同浓度的软枣猕猴桃提取物作用24、48、72小时，采用MTT法评价提取物对细胞增殖的抑制作用，应用流式细胞术检测细胞周期。
结果
观察到提取物对体内外肿瘤均有抑制作用，对S180和H22瘤株的抑制率分别为33.32%和34.62%。提取物可抑制A549细胞在G0-G1期的增殖，并显著降低S期细胞比例。
结论
软枣猕猴桃提取物具有良好的抗肿瘤活性。
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