The Department of Chemistry, Loyola University Chicago, 1068 West Sheridan Road, Chicago, Illinois 60626, United States.
Langmuir. 2010 Dec 7;26(23):18300-2. doi: 10.1021/la103297p. Epub 2010 Nov 3.
A novel approach to pattern silanized-biomolecules directly onto glass (SiO(x)) substrates via Dip-Pen nanolithography (DPN) and microcontact printing (μCP) is presented. Subsequent hybridization reactions of DPN patterned silanized-DNA with its complementary strands provide "proof-of-concept" that the patterned oligonucleotides maintain their biological activities. The fabrication strategy does not require premodification of substrates and offers a cheap and robust way to immobilize molecules on electronically important semiconductor surfaces.
提出了一种通过浸笔纳米光刻（DPN）和微接触印刷（μCP）将图案化硅烷化生物分子直接固定在玻璃（SiO(x)）基底上的新方法。随后，DPN 图案化硅烷化 DNA 与互补链的杂交反应提供了“概念验证”，表明图案化寡核苷酸保持其生物活性。该制造策略不需要对基底进行预修饰，并且为在电子上重要的半导体表面上固定分子提供了一种廉价且稳健的方法。
