Senesi Andrew J, Rozkiewicz Dorota I, Reinhoudt David N, Mirkin Chad A
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
ACS Nano. 2009 Aug 25;3(8):2394-402. doi: 10.1021/nn9005945.
This paper describes a method for the direct transfer of biomolecules encapsulated within a viscous fluid matrix by dip-pen nanolithography (DPN). The method relies on the use of agarose as a "universal" carrier that is compatible with many types of biomolecules including proteins and oligonucleotides. Agarose-assisted DPN allows one to generate nanoarrays of such materials on activated glass substrates with the same deposition rates for different biomolecules, which will greatly expand future capabilities for parallel, multiplexed biomolecule deposition. The fluidity of the matrix may be systematically varied to control the deposition process, resulting in an additional parameter affecting deposition rates besides tip-substrate contact-time and humidity. Agarose-assisted DPN results in extremely fast biomolecule patterning with typical contact times less than 1 s. Feature sizes as small as 50 nm are demonstrated. The biorecognition properties of both protein and oligonucleotide structures are characterized by studying their reactivity with fluorophore-labeled antibody and complementary oligonucleotide sequences, respectively.
本文描述了一种通过蘸笔纳米光刻技术（DPN）直接转移包裹在粘性流体基质中的生物分子的方法。该方法依赖于使用琼脂糖作为“通用”载体，它与包括蛋白质和寡核苷酸在内的多种生物分子兼容。琼脂糖辅助的DPN使人们能够在活化的玻璃基板上生成此类材料的纳米阵列，不同生物分子具有相同的沉积速率，这将极大地扩展未来平行、多重生物分子沉积的能力。基质的流动性可以系统地改变以控制沉积过程，除了针尖-基板接触时间和湿度外，这还导致了另一个影响沉积速率的参数。琼脂糖辅助的DPN能实现极快速的生物分子图案化，典型接触时间小于1秒。展示了小至50纳米的特征尺寸。分别通过研究蛋白质和寡核苷酸结构与荧光团标记抗体及互补寡核苷酸序列的反应性，对它们的生物识别特性进行了表征。
