Protective effects of Phyllanthus amarus on fibrotic markers during alcohol and polyunsaturated fatty acid-induced toxicity.

Alcoholic liver disease (ALD) remains a major problem, with significant morbidity and mortality worldwide. One of the serious consequences of ALD is hepatic fibrosis. This happens when the matrix synthesis rate exceeds that of matrix degradation. Matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) play a key role in matrix remodeling. Disruption of MMP/TIMP balance can lead to excessive accumulation of extracellular matrix components resulting in severe liver injury. The focus of the present study is to analyze the effect of Phyllanthus amarus on MMP and TIMPs activity in alcohol and thermally oxidized polyunsaturated fatty acid (PUFA)-induced hepatic fibrosis. Male albino Wistar rats were used for the study. The matrix metalloproteinase expression was found to be significantly decreased and the levels of TIMPs and the collagen were significantly increased in alcohol + thermally oxidized PUFA-treated rats. Administration of Phyllanthus amarus extract significantly decreased the levels of collagen and TIMPs; and positively modulated the expression of MMPs. From this study, we conclude that Phyllanthus amarus effectively modifies alcohol + thermally oxidized PUFA-induced fibrosis.