Department of Biochemistry and Molecular Biology, Pondicherry University, Kalapet, Puducherry, India.
Eur Rev Med Pharmacol Sci. 2012 Dec;16(15):2147-53.
Alcoholic fibrosis and its end stage cirrhosis represent a major health problem worldwide. Liver fibrosis occurs when the rate of matrix synthesis exceeds matrix degradation. The degree of matrix remodeling depends on the ratio of active matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). The objective of the present work was to study the influence of ferulic acid, a polyphenolic compound, on the expression of MMPs and TIMPs during alcohol and heated polyunsaturated fatty acid (delta PUFA) induced liver toxicity in male albino Wistar rats.
The levels of collagen, the activity of MMPs, the activity of TIMPs, the expression pattern of MMP were analyzed in liver.
The matrix metalloproteinase expression was found to be significantly increased in alcohol as well as delta PUFA treated rats and significantly decreased in alcohol + delta PUFA treated rats. The levels of TIMPs and the collagen were significantly increased in alcohol, delta PUFA and alcohol + delta PUFA groups. Administration of ferulic acid significantly decreased the levels of collagen, TIMPs and positively modulated the expression of MMPs.
Ferulic acid influences MMPs, TIMPs expression and effectively protects liver against alcohol and DPUFA induced liver fibrosis.
酒精性纤维化及其终末期肝硬化是全球范围内的一个主要健康问题。当基质合成速度超过基质降解速度时，就会发生肝纤维化。基质重塑的程度取决于活性基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）的比例。本研究的目的是研究阿魏酸（一种多酚化合物）对雄性白化 Wistar 大鼠酒精和加热多不饱和脂肪酸（delta PUFA）诱导的肝毒性过程中 MMPs 和 TIMPs 表达的影响。
分析肝脏中胶原水平、MMPs 活性、TIMP 活性、MMP 表达模式。
发现 MMP 的表达在酒精以及 delta PUFA 处理的大鼠中显著增加，而在酒精+delta PUFA 处理的大鼠中显著降低。在酒精、delta PUFA 和酒精+delta PUFA 组中，TIMP 水平和胶原含量显著增加。阿魏酸的给药显著降低了胶原、TIMP 的水平，并正向调节了 MMPs 的表达。
阿魏酸影响 MMPs、TIMP 的表达，有效保护肝脏免受酒精和 DPUFA 诱导的肝纤维化。
