LIGBCM, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, Argentina.
J Invertebr Pathol. 2011 Feb;106(2):255-62. doi: 10.1016/j.jip.2010.10.008. Epub 2010 Nov 1.
Dione juno and Agraulis vanillae are very common butterflies in natural gardens in South America, and also bred worldwide. In addition, larvae of these butterflies are considered as pests in crops of Passiflora spp. For these reasons, it is important to identify and describe pathogens of these species, both for preservation purposes and for use in pest control. Baculoviridae is a family of insect viruses that predominantly infect species of Lepidoptera and are used as bioinsecticides. Larvae of D. juno and A. vanillae exhibiting symptoms of baculovirus infection were examined for the presence of baculoviruses by PCR and transmission electron microscopy. Degenerate primers were designed and used to amplify partial sequences from the baculovirus p74, cathepsin, and chitinase genes, along with previously designed primers for amplification of lef-8, lef-9, and polh. Sequence data from these six loci, along with ultrastructural observations on occlusion bodies isolated from the larvae, confirmed that the larvae were infected with nucleopolyhedroviruses from genus Alphabaculovirus. The NPVs from the two different larval hosts appear to be variants of the same, previously undescribed baculovirus species. Phylogenetic analysis of the sequence data placed these NPVs in Alphabaculovirus group I/clade 1b.
戴娜姬娜蝶和香草波淡眼蝶是南美的自然花园中非常常见的蝴蝶，也在全球范围内进行养殖。此外，这些蝴蝶的幼虫被认为是 Passiflora spp. 作物的害虫。出于这些原因，识别和描述这些物种的病原体非常重要，既用于保护目的，也用于害虫控制。杆状病毒科是一类主要感染鳞翅目昆虫的昆虫病毒，被用作生物杀虫剂。表现出杆状病毒感染症状的 D. juno 和 A. vanillae 幼虫通过 PCR 和透射电子显微镜检查杆状病毒的存在。设计了简并引物，用于扩增杆状病毒 p74、组织蛋白酶和几丁质酶基因的部分序列，以及先前设计的用于扩增 lef-8、lef-9 和 polh 的引物。来自这六个基因座的序列数据，以及从幼虫中分离出的包埋体的超微结构观察，证实幼虫感染了来自 Alphabaculovirus 属的核多角体病毒。来自两种不同幼虫宿主的 NPV 似乎是同一先前未描述的杆状病毒种的变体。序列数据分析将这些 NPV 置于 Alphabaculovirus 组 I/clade 1b 中。
