van Oers Monique M, Herniou Elisabeth A, Usmany Magda, Messelink Gerben J, Vlak Just M
Laboratory of Virology, Wageningen University, 6709 PD Wageningen, The Netherlands.
Virology. 2004 Dec 20;330(2):460-70. doi: 10.1016/j.virol.2004.09.032.
A hitherto unknown single nucleocapsid nucleopolyhedrovirus (SNPV) with a unique property was isolated from larvae of the looper Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). Polyhedrin, lef-8, and pif-2 gene sequences were obtained by PCR with degenerate primers and used for phylogenetic analysis. ChchNPV belonged to class II NPVs and its polyhedrin sequence was most similar to that of class II NPVs of other members of the subfamily Plusiinae. Further genetic characterization involved the random cloning of HindIII fragments into a plasmid vector and analysis by end-in sequencing. A gene so far unique to baculoviruses was identified, which encodes a putative DNA repair enzyme: cyclobutane pyrimidine dimer (CPD) DNA photolyase (dpl). The transcriptional activity of this gene was demonstrated in both ChchNPV-infected C. chalcites larvae and infected Trichoplusia ni High Five cells by RT-PCR and 5' and 3' RACE analysis. The possible role of this gene in the biology of the virus is discussed.
从斜纹夜蛾（鳞翅目，夜蛾科，金翅夜蛾亚科）幼虫中分离出一种具有独特特性的此前未知的单核衣壳核型多角体病毒（SNPV）。通过使用简并引物进行PCR获得了多角体蛋白、lef - 8和pif - 2基因序列，并用于系统发育分析。ChchNPV属于II类NPV，其多角体蛋白序列与金翅夜蛾亚科其他成员的II类NPV最为相似。进一步的基因特征分析包括将HindIII片段随机克隆到质粒载体中，并通过末端测序进行分析。鉴定出一种迄今为止杆状病毒特有的基因，该基因编码一种假定的DNA修复酶：环丁烷嘧啶二聚体（CPD）DNA光解酶（dpl）。通过RT - PCR以及5'和3' RACE分析，在ChchNPV感染的斜纹夜蛾幼虫和感染的粉纹夜蛾High Five细胞中均证明了该基因的转录活性。讨论了该基因在病毒生物学中的可能作用。
