使用Luminex™多重技术进行HLA基因分型和抗体鉴定
HLA Genotyping and Antibody Characterization Using the Luminex™ Multiplex Technology.
作者信息
Heinemann Falko Markus
机构信息
Institute for Transfusion Medicine, University Hospital Essen, Germany.
出版信息
Transfus Med Hemother. 2009;36(4):273-278. doi: 10.1159/000228834. Epub 2009 Jul 21.
Abstract
The Luminex-based human leukocyte antigen (HLA) antibody screening technology is widespread used in laboratories affiliated to kidney transplantation programs and enables both screening (i.e. the definition of positive or negative antibody status) and antibody identification with high sensitivity and specificity. HLA typing at different levels of resolution with Luminex technology uses sequence-specific oligonucleotide probes bound to color-coded microbeads in order to identify HLA alleles encoded by the DNA sample. In general, the Luminex technology for histocompatibility analyses provides rapid sample processing in a 96-well format combined with limited technical complexity which means high cost efficiency.
摘要
基于Luminex的人类白细胞抗原（HLA）抗体筛查技术在肾移植项目所属实验室中广泛应用，能够以高灵敏度和特异性进行筛查（即确定抗体状态为阳性或阴性）以及抗体鉴定。使用Luminex技术进行不同分辨率水平的HLA分型时，会利用与彩色编码微珠结合的序列特异性寡核苷酸探针来识别DNA样本编码的HLA等位基因。一般来说，用于组织相容性分析的Luminex技术以96孔板形式提供快速样本处理，且技术复杂性有限，这意味着成本效益高。
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