Centre for Women's Health Research, Monash Institute of Medical Research and Monash University Department of Obstetrics and Gynaecology, Monash Medical Centre, 246 Clayton Rd, Clayton, Victoria 3168, Australia.
Reprod Biomed Online. 2010 Dec;21(6):803-11. doi: 10.1016/j.rbmo.2010.07.005. Epub 2010 Jul 18.
While vascular endothelial growth factor (VEGF)-A mediates endometrial vascular remodelling during early pregnancy in mice, individual VEGF-A isoforms have not been investigated, despite their different biological properties. Using mice as a model, the expression of VEGF-A isoforms and receptors in the mouse uterus during early pregnancy was quantified. It was postulated that selected isoform expression would increase concurrent with increased endometrial endothelial cell proliferation at this time. Uteri were collected on days 1-5 of pregnancy and mRNA expression was quantified by quantitative reverse-transcription polymerase chain reaction, VEGF-A protein by Western blot and VEGF receptor (VEGFR)-2 by immunohistochemistry. The lowest expression of isomers Vegf(120) and Vegf(164) was observed on day 2 of pregnancy, increasing thereafter. Vegfr-2 mRNA expression was significantly higher on days 3-5 of pregnancy relative to days 1-2 (P<0.05). No significant changes were noted in Vegf(188), Nrp1 or Nrp2 mRNA. VEGF(188) protein expression was consistently higher than other isoforms. These data demonstrate differential regulation of VEGF-A isoforms in mouse uterus during early pregnancy.
虽然血管内皮生长因子 (VEGF)-A 在小鼠早孕期间介导子宫内膜血管重塑，但尽管其具有不同的生物学特性，但尚未研究其各个亚型。本研究使用小鼠作为模型，定量检测了小鼠子宫在早孕期间的 VEGF-A 亚型和受体的表达。假设在此期间，随着子宫内膜内皮细胞增殖的增加，选定的亚型表达会增加。在妊娠第 1-5 天收集子宫，通过定量逆转录聚合酶链反应定量 mRNA 表达，通过 Western blot 检测 VEGF-A 蛋白，通过免疫组织化学检测血管内皮生长因子受体 (VEGFR)-2。在妊娠第 2 天观察到异构体 Vegf(120)和 Vegf(164)的表达最低，此后增加。与第 1-2 天相比，妊娠第 3-5 天的 Vegfr-2 mRNA 表达显著更高（P<0.05）。Vegf(188)、Nrp1 或 Nrp2 mRNA 无明显变化。VEGF(188)蛋白表达始终高于其他亚型。这些数据表明，在小鼠早孕期间，VEGF-A 亚型在子宫中的调节存在差异。
