Harrison S A, Mondino B J, Kagan J M
Jules Stein Eye Institute, Department of Ophthalmology, Los Angeles, CA 90024.
Invest Ophthalmol Vis Sci. 1990 Jan;31(1):163-7.
We investigated the ability of recombinant human gamma-interferon (rhIFN-gamma) to induce class II HLA antigen expression on human conjunctival fibroblasts in cell culture. Cultures were established by explanting subconjunctival tissue from normal donor globes. Fibroblasts were treated with rhIFN-gamma at concentrations ranging from 1 to 500 units/ml and incubated for 1, 3 and 6 days. HLA antigens were detected by immunofluorescence using monoclonal antibodies in conjunction with flow cytometry. Class I antigen was identified using a monoclonal antibody directed against Beta-2 microglobulin (a component of the class I antigen complex). Class II histocompatibility antigens were detected using monoclonal antibodies specific for HLA-DR, HLA-DP and HLA-DQ. Class I antigen was present on all cells prior to induction and showed a trend toward increased density after treatment with rhIFN-gamma. Class II antigens were absent before induction with rhIFN-gamma. After treatment with rhIFN-gamma, class II antigens were induced in a dose- and time-dependent fashion. HLA-DR expression was most sensitive to induction by rhIFN-gamma, followed by HLA-DP, and then HLA-DQ. The up-regulation of HLA class I antigen expression and the inducible expression of class II antigens following exposure to rhIFN-gamma suggest that conjunctival fibroblasts have the potential to participate in immunologic diseases of the external eye.
我们研究了重组人γ干扰素（rhIFN-γ）在细胞培养中诱导人结膜成纤维细胞表达Ⅱ类HLA抗原的能力。通过从正常供体眼球的结膜下组织取材建立培养物。用浓度范围为1至500单位/毫升的rhIFN-γ处理成纤维细胞，并孵育1、3和6天。使用单克隆抗体结合流式细胞术通过免疫荧光检测HLA抗原。使用针对β2微球蛋白（Ⅰ类抗原复合物的一个成分）的单克隆抗体鉴定Ⅰ类抗原。使用对HLA-DR、HLA-DP和HLA-DQ特异的单克隆抗体检测Ⅱ类组织相容性抗原。在诱导之前，所有细胞上都存在Ⅰ类抗原，在用rhIFN-γ处理后，其密度有增加的趋势。在用rhIFN-γ诱导之前，Ⅱ类抗原不存在。用rhIFN-γ处理后，Ⅱ类抗原以剂量和时间依赖性方式被诱导。HLA-DR表达对rhIFN-γ诱导最敏感，其次是HLA-DP，然后是HLA-DQ。暴露于rhIFN-γ后HLAⅠ类抗原表达的上调和Ⅱ类抗原的可诱导表达表明结膜成纤维细胞有可能参与外眼的免疫疾病。
