Calonge M C, Pastor J C, Herreras J M, González J L
Department of Ophthalmology, Valladolid Medical School, Spain.
Invest Ophthalmol Vis Sci. 1990 Jan;31(1):176-80.
Evans blue (EB) dye extravasation has been used as a reliable and objective parameter of the increased vascular permeability of an allergic conjunctivitis experimental rat model that closely mimics human ocular allergy. Five male Wistar rats, previously immunized (Group 1), had DL-dithiothreitol (DTT) applied topically to one eye 15 min prior to topical challenge with egg albumin (EA). The fellow eye (control) received phosphate buffered saline (PBS) 15 min prior to receiving EA. Immediately prior to challenge, the rats were injected intravenously with EB. After 30 min, the animals were killed and the dye extracted from the eyes. The intensity of EB extravasation was determined by spectrophotometry at 620 nm. EB extravasation was significantly higher in the eyes that received DTT than in those that received PBS. Groups 2, 3 and 4 of nonimmunized rats served as additional controls: Group 2 for DTT toxicity, Group 3 as a proof of the reaginic antibody mediation and Group 4 as a control of EB extravasation under normal conditions. Five additional groups of five rats each were immunized and both eyes of each rat received DTT 15 min before EA challenge. One eye of each rat received 0.1% dexamethasone sodium phosphate topically (Group 5), 0.1% pyrilamine maleate (Groups 6 and 7), and 2% disodium cromoglycate (DSCG) (Groups 8 and 9). The fellow eye received the solvent of each drug topically (control). In the eyes treated with antiallergic drugs, EB extravasation decreased 40% for dexamethasone, 44.1% and 10.4% for pyrilamine, and 51.4% and 51.2% for DSCG.
伊文思蓝（EB）染料外渗已被用作一种可靠且客观的参数，用于评估过敏性结膜炎实验大鼠模型中血管通透性增加的情况，该模型与人类眼部过敏极为相似。5只先前已免疫的雄性Wistar大鼠（第1组），在局部用卵清蛋白（EA）激发前15分钟，将DL-二硫苏糖醇（DTT）局部应用于一只眼睛。另一只眼睛（对照）在接受EA前15分钟接受磷酸盐缓冲盐水（PBS）。在激发前即刻，给大鼠静脉注射EB。30分钟后，处死动物并从眼睛中提取染料。通过在620nm处的分光光度法测定EB外渗的强度。接受DTT的眼睛中EB外渗明显高于接受PBS的眼睛。未免疫的大鼠第2、3和4组用作额外对照：第2组用于评估DTT毒性，第3组用于验证反应素抗体介导作用，第4组用于作为正常条件下EB外渗的对照。另外五组，每组五只大鼠，进行免疫，每只大鼠的双眼在EA激发前15分钟接受DTT。每只大鼠的一只眼睛局部接受0.1%地塞米松磷酸钠（第5组）、0.1%马来酸氯苯那敏（第6和7组）以及2%色甘酸钠（DSCG）（第8和9组）。另一只眼睛局部接受每种药物的溶剂（对照）。在用抗过敏药物治疗的眼睛中，地塞米松使EB外渗减少40%，马来酸氯苯那敏分别减少44.1%和10.4%，色甘酸钠分别减少51.4%和51.2%。
