比较玻璃体内庆大霉素、加替沙星和莫西沙星对兔模型角膜内皮的影响。
Comparison of the influence of intracameral gentamicin, gatifloxacin, and moxifloxacin on the corneal endothelium in a rabbit model.
机构信息
Department of Ophthalmology, School of Medicine, Toho University, Tokyo, Japan.
出版信息
Jpn J Ophthalmol. 2010 Sep;54(5):481-5. doi: 10.1007/s10384-010-0838-5. Epub 2010 Nov 5.
PURPOSE
To compare the effect of three intracameral antibiotics, gentamicin (GM), gatifloxacin (GFLX), and moxifloxacin (MFLX), on the rabbit corneal endothelium.
METHODS
Twenty-four eyes from 18 rabbits were used. In the GM treatment group of 12 eyes, a dose of 20 mg/ml, 2 mg/ml, 200 μg/ml, or 20 μg/ml of GM was injected into the anterior chamber. In the GFLX and MFLX treatment groups were injected into the anterior chamber of three eyes. The central corneal thickness was measured. The eyes were then enucleated for observation under scanning electron microscopy.
RESULTS
Three days after the intracameral injection, a significant difference in central corneal thickness was found between the GM 20 mg/ml group and the control group (P < 0.05), but not between any other groups. The damage rate at the endothelial cell level was 67% in the GM 20 mg/ml group, 56% in the GM 2 mg/ml group, 33% in the GM 200 μg/ml group, 22% in the GM 20 μg/ml group, 22% in the GFLX group, and 0% in the MFLX group.
CONCLUSIONS
Intracameral GFLX or MFLX was almost nontoxic to the rabbit corneal endothelium, in contrast to the toxic results of intracameral GM 20 and 2 mg/ml.
目的
比较三种眼内抗生素（庆大霉素[GM]、加替沙星[GFLX]和莫西沙星[MFLX]）对兔角膜内皮的影响。
方法
18 只兔子的 24 只眼参与本研究。在 GM 治疗组的 12 只眼中，将 20mg/ml、2mg/ml、200μg/ml 或 20μg/ml 的 GM 剂量注入前房。在 GFLX 和 MFLX 治疗组中，向三只眼的前房内注入三种药物。测量中央角膜厚度。然后，对眼球进行眼球摘出，并在扫描电子显微镜下进行观察。
结果
在眼内注射后 3 天，GM 20mg/ml 组与对照组的中央角膜厚度存在显著差异（P<0.05），但其他组之间无差异。GM 20mg/ml 组的内皮细胞损伤率为 67%，GM 2mg/ml 组为 56%，GM 200μg/ml 组为 33%，GM 20μg/ml 组为 22%，GFLX 组为 22%，MFLX 组为 0%。
结论
与 GM 20 和 2mg/ml 相比，GFLX 或 MFLX 几乎对兔角膜内皮无毒性，而 GM 20 和 2mg/ml 则具有毒性。
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