Rivinoja Antti, Laakkonen Pirjo
Molecular Cancer Biology Research Program and Institute of Biomedicine, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland.
Methods Mol Biol. 2011;683:401-15. doi: 10.1007/978-1-60761-919-2_29.
Each normal organ and pathological condition expresses a distinct set of molecules on their vasculature. These molecular signatures have been efficiently profiled using in vivo phage display technology. Using this technology, several peptides homing specifically to tumour blood vessels, lymphatic vessels, and/or tumour cells as well as to various normal organs have been isolated. Peptides homing to specific vascular addresses have revealed novel tissue-specific biomarkers of the normal and diseased vasculature. Tumour homing peptides have been successfully used to target therapies and imaging agents to tumours. In this review, we describe experimental setup for a combined ex vivo and in vivo screening procedure to select peptides homing to tumours.
每个正常器官和病理状况在其脉管系统上都表达一组独特的分子。利用体内噬菌体展示技术已有效地描绘出了这些分子特征。运用该技术，已分离出几种分别特异性归巢于肿瘤血管、淋巴管和/或肿瘤细胞以及各种正常器官的肽。归巢于特定血管位点的肽揭示了正常和患病脉管系统新的组织特异性生物标志物。肿瘤归巢肽已成功用于将治疗药物和成像剂靶向输送至肿瘤。在本综述中，我们描述了一种用于选择肿瘤归巢肽的体外和体内联合筛选程序的实验设置。
