基于随机扩增多态性DNA（RAPD）标记的选定土耳其桑树基因型（桑属）之间的遗传关系

Genetic relationships between selected Turkish mulberry genotypes (Morus spp) based on RAPD markers.

作者信息

Orhan E, Ercisli S

机构信息

Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, Erzurum, Turkey.

出版信息

Genet Mol Res. 2010 Nov 3;9(4):2176-83. doi: 10.4238/vol9-4gmr958.

DOI:10.4238/vol9-4gmr958

PMID:21053181

Abstract

Mulberry (Morus spp, Moraceae) is an important horticultural crop in Turkey, which is one of the main world producers of mulberry fruit. We evaluated the genetic relationships among 26 mulberry genotypes selected for agronomic characteristics, using RAPD markers. A total of 367 DNA markers were generated with 34 random primers. The highest genetic similarity (0.80) was observed between Oltu58 (M. nigra) and Olur90 (M. nigra) genotypes. The genotypes Oltu3 (M. alba) and Oltu18 (M. rubra) were the most distant (0.36). We found that the RAPD technique is a useful tool to discriminate mulberry genotypes at both the intra- and interspecific level. This type of information will aid in accurate identification of useful genotypes for breeding programs.

摘要

桑树（桑属，桑科）是土耳其一种重要的园艺作物，该国是世界主要的桑椹生产国之一。我们使用随机扩增多态性DNA（RAPD）标记评估了26个因农艺性状而挑选出的桑树基因型之间的遗传关系。34个随机引物共产生了367个DNA标记。在奥尔图58（黑桑）和奥卢尔90（黑桑）基因型之间观察到最高的遗传相似性（0.80）。奥尔图3（白桑）和奥尔图18（红桑）基因型的亲缘关系最远（0.36）。我们发现RAPD技术是在种内和种间水平鉴别桑树基因型的有用工具。这类信息将有助于准确鉴定育种计划中的有用基因型。