利用 RAPD 和 ISSR 对桑树种质进行分子特征分析。

Molecular characterization of mulberry (Morus spp.) genotypes via RAPD and ISSR.

机构信息

Department of Horticulture, Faculty of Agriculture, Selçuk University, Konya, Turkey.

出版信息

J Sci Food Agric. 2012 Jun;92(8):1633-7. doi: 10.1002/jsfa.4749. Epub 2011 Dec 20.

DOI:10.1002/jsfa.4749

PMID:22222844

Abstract

BACKGROUND

In recent years, DNA-based markers have been used quite extensively because of their many advantages over the traditional morphological and biochemical markers. Many studies have shown that molecular markers are useful in delineating the genetic relationships among closely related mulberry genotypes and cultivars. Thus, in the present study, polymer chain reaction based DNA fingerprinting techniques were used to investigate the genetic relationships among mulberry genotypes growing in different agro-climatic regions of Turkey.

RESULTS

20 RAPD primers generated a total of 173 bands, of which 157 (90.75%) were polymorphic. As for 11 ISSR primers, 124 bands (96.55%) were polymorphic in a total of 128. The similarity index for RAPD technique ranged between 0.24-0.98; 25İs203 with 25İs112 were found to be the closest genotypes, while 24Ke10 and 25İs123 were the most distant ones. According to the ISSR result, the genetic similarity index changed between 0.21-095; 25İs203 with 25İs112 genotypes were the closest, while 25İs08 and 01KaD2 were the most distant ones.

CONCLUSION

The RAPD and ISSR markers were found to be promising for assessing genetic diversity in mulberry genotypes.

摘要

背景

近年来，由于 DNA 标记物具有许多优于传统形态学和生化标记物的优点，因此被广泛应用。许多研究表明，分子标记物在描绘亲缘关系密切的桑树基因型和品种之间的遗传关系方面非常有用。因此，本研究采用基于聚合酶链反应的 DNA 指纹技术，研究了土耳其不同农业气候区生长的桑树基因型之间的遗传关系。

结果

20 个 RAPD 引物共产生 173 条带，其中 157 条（90.75%）为多态性。对于 11 个 ISSR 引物，总共 128 条中有 124 条（96.55%）为多态性。RAPD 技术的相似性指数范围为 0.24-0.98；25İs203 与 25İs112 是最接近的基因型，而 24Ke10 和 25İs123 是最不接近的。根据 ISSR 结果，遗传相似性指数在 0.21-0.95 之间变化；25İs203 与 25İs112 基因型最接近，而 25İs08 和 01KaD2 最不接近。