链霉菌属物种的基因操作。
Genetic manipulation of Streptomyces species.
作者信息
Nybo S Eric, Shepherd Micah D, Bosserman Mary A, Rohr Jürgen
机构信息
College of Pharmacy, University of Kentucky, Lexington, Kentucky, USA.
出版信息
Curr Protoc Microbiol. 2010 Nov;Chapter 10:Unit 10E.3. doi: 10.1002/9780471729259.mc10e03s19.
Abstract
This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR.
摘要
本单元包括链霉菌属物种基因操作的一般方案，包括基因组DNA分离、基因组文库制备、链霉菌与大肠杆菌的属间接合、链霉菌原生质体的产生和转化、链霉菌菌丝体的电穿孔以及菌落PCR。
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A Simple and Rapid Method of Transformation of Streptomyces rimosus R6 and Other Streptomycetes by Electroporation.通过电穿孔转化瑞氏木霉 R6 和其他链霉菌的简单快速方法。
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