用单克隆抗体通过抗原检测诊断星状病毒肠胃炎。

Diagnosis of astrovirus gastroenteritis by antigen detection with monoclonal antibodies.

作者信息

Herrmann J E, Nowak N A, Perron-Henry D M, Hudson R W, Cubitt W D, Blacklow N R

机构信息

Division of Infectious Diseases, University of Massachusetts Medical School, Worcester 01655.

出版信息

J Infect Dis. 1990 Feb;161(2):226-9. doi: 10.1093/infdis/161.2.226.

DOI:10.1093/infdis/161.2.226

PMID:2105359

Abstract

An enzyme-linked immunosorbent assay (ELISA), based on monoclonal antibodies to the astrovirus group antigen, was designed for the detection of astroviruses in stools of patients with gastroenteritis. Compared to immune electron microscopy used as the standard test, the sensitivity of the astrovirus ELISA was 91% (31/34) and the specificity was 96% (54/56). All five of the known astrovirus serotypes could be detected in 16 samples on which serotyping was done. In tests on 155 stools containing other enteric viruses, including adenoviruses, rotaviruses, caliciviruses, Hawaii virus, Snow Mountain virus, and Norwalk virus (30, 20, 70, 24, 4, and 7 samples, respectively), only 3 were positive in the astrovirus ELISA. The combined specificity for all astrovirus immune electron microscopy-negative samples was 98% (206/211). The results demonstrate that the new ELISA provides a sensitive and specific means for the diagnosis of astrovirus gastroenteritis.

摘要

基于抗星状病毒群抗原单克隆抗体的酶联免疫吸附测定（ELISA）被设计用于检测胃肠炎患者粪便中的星状病毒。与用作标准检测方法的免疫电子显微镜相比，星状病毒ELISA的灵敏度为91%（31/34），特异性为96%（54/56）。在进行血清分型的16个样本中，可以检测到所有5种已知的星状病毒血清型。在对155份含有其他肠道病毒的粪便进行检测时，包括腺病毒、轮状病毒、杯状病毒、夏威夷病毒、雪山病毒和诺沃克病毒（分别为30、20、70、24、4和7个样本），星状病毒ELISA检测中只有3份呈阳性。所有星状病毒免疫电子显微镜检测阴性样本的综合特异性为98%（206/211）。结果表明，新的ELISA为星状病毒胃肠炎的诊断提供了一种灵敏且特异的方法。