Pérot Philippe, Lecuit Marc, Eloit Marc
Institut Pasteur, Biology of Infection Unit, Inserm U1117, Laboratory of Pathogen Discovery, 75015 Paris, France.
Institut Pasteur, Centre d'innovation et de Recherche Technologique (Citech), 75015 Paris, France.
Viruses. 2017 Jan 12;9(1):10. doi: 10.3390/v9010010.
Various methods exist to detect an astrovirus infection. Current methods include electron microscopy (EM), cell culture, immunoassays, polymerase chain reaction (PCR) and various other molecular approaches that can be applied in the context of diagnostic or in surveillance studies. With the advent of metagenomics, novel human astrovirus (HAstV) strains have been found in immunocompromised individuals in association with central nervous system (CNS) infections. This work reviews the past and current methods for astrovirus detection and their uses in both research laboratories and for medical diagnostic purposes.
存在多种检测星状病毒感染的方法。目前的方法包括电子显微镜（EM）、细胞培养、免疫测定、聚合酶链反应（PCR）以及可应用于诊断或监测研究的各种其他分子方法。随着宏基因组学的出现，在免疫功能低下个体中发现了与中枢神经系统（CNS）感染相关的新型人类星状病毒（HAstV）毒株。本文综述了过去和当前用于星状病毒检测的方法及其在研究实验室和医学诊断中的应用。
