State Key Laboratory of Pollution Control and Resources Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Environ Sci Technol. 2010 Dec 1;44(23):8957-63. doi: 10.1021/es102547n. Epub 2010 Nov 5.
Wastewater biological nutrient removal (BNR) by short-cut nitrification-denitrification (SCND) and denitrifying phosphorus removal via nitrite (DPRN) has several advantages, such as organic carbon source saving. In this paper, a new method, i.e., by using waste activated sludge alkaline fermentation liquid as BNR carbon source, for simultaneously improving SCND and DPRN was reported. First, the performance of SCND and DPRN with sludge fermentation liquid as carbon source was compared with acetic acid, which was commonly used in literatures. Sludge fermentation liquid showed much higher nitrite accumulation during aerobic nitrification than acetic acid (81.8% versus 40.9%), and the former had significant anoxic denitrification and phosphorus uptake. The soluble phosphorus and total nitrogen removal efficiencies with sludge fermentation liquid were much higher than with acetic acid (97.6% against 73.4% and 98.7% versus 79.2%). Then the mechanisms for sludge fermentation liquid showed higher SCND and DPRN than acetic acid were investigated from the aspects of wastewater composition, microorganisms assayed by 16S rRNA gene clone library, and fluorescence in situ hybridization. More NO(2)(-)-N accumulated by the use of sludge fermentation liquid was attributed to be more humic acids in the influent, which inhibited nitrite oxidizing bacteria (NOB) more serious than ammonia oxidizing bacteria (AOB), and more AOB but less NOB were observed in the BNR system. The reasons for sludge fermentation liquid BNR system exhibiting greater short-cut denitrifying phosphorus removal were that there were less glycogen accumulating organisms and more phosphorus accumulating organisms and anoxic denitrifying phosphorus removal bacteria with higher nitrite reductase activity.
污水短程硝化-反硝化生物脱氮（SCND）和亚硝酸盐反硝化除磷（DPRN）具有节省有机碳源等优点。本文报道了一种利用剩余污泥碱性发酵液作为 SCND 和 DPRN 反硝化碳源的新方法，以同时提高 SCND 和 DPRN 的性能。首先，比较了以污泥发酵液和乙酸作为碳源的 SCND 和 DPRN 的性能，乙酸是文献中常用的碳源。污泥发酵液在好氧硝化过程中积累的亚硝酸盐明显高于乙酸（81.8%比 40.9%），且具有较强的缺氧反硝化和吸磷能力。以污泥发酵液为碳源时，溶解性磷和总氮的去除效率明显高于乙酸（97.6%比 73.4%和 98.7%比 79.2%）。然后，从废水成分、16S rRNA 基因克隆文库分析的微生物以及荧光原位杂交等方面，探讨了污泥发酵液具有较高 SCND 和 DPRN 的原因。污泥发酵液中积累了更多的 NO(2)(-)-N，这归因于进水有机物中含有更多的腐殖酸，腐殖酸对亚硝酸氧化菌（NOB）的抑制作用比对氨氧化菌（AOB）更严重，而且在 SCND 系统中观察到更多的 AOB 和更少的 NOB。污泥发酵液 BNR 系统表现出更大的短程反硝化除磷能力的原因是，系统中聚磷菌和反硝化除磷菌的数量较多，而具有较高亚硝酸盐还原酶活性的聚糖菌数量较少。
