College of Civil Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.
Key Laboratory of Biotherapy and Regenerative Medicine Transformation Gansu Province, The First Hospital of Lanzhou University, Lanzhou, 730030, China.
Arch Microbiol. 2024 Oct 19;206(11):439. doi: 10.1007/s00203-024-04158-2.
Previous research on Magnetospirillum gryphiswaldense MSR-1 found that MSR-1 has a good denitrification nitrogen removal ability and specific application prospects in the sewage biological nitrogen removal field. Therefore, this study selected the essential denitrification gene NapA in MSR-1-wt for overexpression, and the overexpressed MSR-1-NapA was successfully constructed. Q-PCR amplification experiment and AGAR gel electrophoresis experiment proved that the relative transcription level of the NapA gene was increased by more than four times, and the denitrification ability of MSR-1-wt and MSR-1-NapA was further determined by enzyme activity experiment, denitrification experiment, and flow cytometry. The results showed that overexpression of the NapA gene increased nitrate reductase activity in MSR-1-NapA by more than four times. In the solution with a nitrate concentration of 118.33 ± 3.23 mgN/L, the denitrification efficiency of MSR-1-NapA was superior to that of MSR-1-wt, significantly enhancing both the denitrification and nitrogen removal capacities of MSR-1. This indicates its greater potential for biological nitrogen removal in wastewater treatment.
先前对嗜中性灵杆菌 MSR-1 的研究发现，MSR-1 具有良好的脱氮除氮能力，在污水生物脱氮领域具有特定的应用前景。因此，本研究选择 MSR-1-wt 中的必需脱氮基因 NapA 进行过表达，成功构建了过表达 MSR-1-NapA。Q-PCR 扩增实验和 AGAR 凝胶电泳实验证明，NapA 基因的相对转录水平提高了四倍以上，进一步通过酶活性实验、脱氮实验和流式细胞术确定了 MSR-1-wt 和 MSR-1-NapA 的脱氮能力。结果表明，NapA 基因的过表达使 MSR-1-NapA 中的硝酸还原酶活性提高了四倍以上。在硝酸盐浓度为 118.33±3.23mgN/L 的溶液中，MSR-1-NapA 的脱氮效率优于 MSR-1-wt，显著增强了 MSR-1 的脱氮和除氮能力。这表明其在污水处理中具有更大的生物脱氮潜力。
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