Bai Feng, Li Hai-qi
Department of Primary Child Care, Children's Hospital, Chongqing Medical University, Chongqing 400014, China.
Zhonghua Er Ke Za Zhi. 2010 Jul;48(7):520-5.
To investigate the effects of fulvotomentoside (Ful) on inflammatory factors and antiinflammatory factors in intestine of ovalbumin (OVA)-sensitized BALB/c mice, and to explore the mechanisms of its anti-food allergy effect.
Twenty-four female BALB/c mice aged 6 weeks fed with ovalbumin-free feed were randomly divided into 3 groups, food allergy (FA) group, Ful group and normal saline (NS) group. Mice in FA and Ful groups were sensitized intraperitoneally two times with OVA and challenged intragastrically with OVA. Mice in Ful group were treated with 200 mg/kg of Ful by subcutaneous injection once daily for 22 days. The mice in FA and NS groups were used as positive control and negative control, respectively, and were treated with normal saline solution by subcutaneous injection for 22 days. Just 48 hours after the last challenge, the mice in each group were sacrificed and specimens of jejunum were taken. The mRNA expressions of transforming growth factor β1 (TGF-β1), interleukin-6 (IL-6), interleukin-17A (IL-17A) and forkhead box P3 (Foxp3) in jejunum were detected by reverse transcription-PCR (RT-PCR). The protein expressions of TGF-β1, IL-6, and IL-17A in jejunum were detected by immunohistochemical method. The activation of neutrophils in jejunum was assayed by the levels of MPO.
The expressions of TGF-β1, IL-6, IL-17A mRNA [(0.370 ± 0.013), (0.475 ± 0.015), (0.541 ± 0.013)] and related protein [(53,075.70 ± 20,727.06), (256,881.66 ± 36,561.79), (435,064.25 ± 69,911.48)] in jejunum were increased and the Foxp3 mRNA [(0.231 ± 0.014) vs. (0.365 ± 0.015)] expression was decreased in group FA. After the treatment with Ful, IL-6 and IL-17A mRNA [(0.196 ± 0.005), (0.204 ± 0.008)] and protein [(114,040.30 ± 20,295.25), (218,200.74 ± 30,077.69)] expressions were decreased and Foxp3 mRNA (0.578 ± 0.021) expression was increased, and no change of TGF-β1 expression was unchanged. There were no significant differences of the levels of MPO among the three groups.
Inflammatory reaction which was characterized by the increase of IL-6 and IL-17A expressions was found in intestine of ovalbumin-sensitized BALB/c mice. Ful could decrease overexpression of IL-6 and IL-17A, and increase the expression of specific transcription factor Foxp3 of regulatory T cells significantly in intestine. It may be one of the mechanisms that Ful improved intestinal inflammation.
探讨富伏托门糖苷（Ful）对卵清蛋白（OVA）致敏的BALB/c小鼠肠道炎症因子和抗炎因子的影响，探讨其抗食物过敏作用机制。
将24只6周龄无卵清蛋白饲料喂养的雌性BALB/c小鼠随机分为3组，食物过敏（FA）组、Ful组和生理盐水（NS）组。FA组和Ful组小鼠腹腔注射OVA致敏2次，灌胃OVA激发。Ful组小鼠皮下注射200mg/kg Ful，每日1次，共22天。FA组和NS组小鼠分别作为阳性对照和阴性对照，皮下注射生理盐水22天。末次激发后48小时，处死各组小鼠，取空肠标本。采用逆转录-聚合酶链反应（RT-PCR）检测空肠中转化生长因子β1（TGF-β1）、白细胞介素-6（IL-6）、白细胞介素-17A（IL-17A）和叉头框P3（Foxp3）的mRNA表达。采用免疫组织化学方法检测空肠中TGF-β1、IL-6和IL-17A的蛋白表达。通过髓过氧化物酶（MPO）水平检测空肠中性粒细胞的活化情况。
FA组空肠中TGF-β1、IL-6、IL-17A mRNA表达[（0.370±0.013），（0.475±0.015），（0.541±0.013）]及相关蛋白表达[（53075.70±20727.06），（256881.66±36561.79），（435064.25±69911.48）]升高，Foxp3 mRNA表达[（0.231±0.014）对（0.365±0.015）]降低。Ful治疗后，IL-6和IL-17A mRNA表达[（0.196±0.005），（0.204±0.008）]及蛋白表达[（114040.30±20295.25），（218200.74±30077.69）]降低，Foxp3 mRNA表达（0.578±0.021）升高，TGF-β1表达无变化。三组MPO水平差异无统计学意义。
卵清蛋白致敏的BALB/c小鼠肠道存在以IL-6和IL-17A表达增加为特征的炎症反应。Ful可降低肠道中IL-6和IL-17A的过表达，显著增加调节性T细胞特异性转录因子Foxp3的表达。这可能是Ful改善肠道炎症的机制之一。
