Bai Feng, Li Hai-qi
Department of Primary Child Care, Children's Hospital, Chongqing Medical University, Chongqing, China.
Zhonghua Er Ke Za Zhi. 2012 Jul;50(7):531-5.
To investigate immune state in lung of BALB/c mice with ovalbumin (OVA) allergy and the effects of fulvotomentoside (Ful) on lungs of the mice and provide some clues for the mechanism that patients with food allergies were prone to asthma and observe the effects of the treatment with traditional Chinese medicine.
Ninety-six female BALB/c mice were randomly divided into 6 groups. Mice in group 1 and group 2 were sensitized intraperitoneally and challenged intragastrically with OVA and were exposed to phosphate buffer solution and OVA respectively by nebulized inhalation. Mice in group 3 and group 4 were treated with Ful, other processes were the same as the mice in group 1 and group 2, respectively. Mice in group 5 were not challenged intragastrically with OVA and other processes were the same as the mice in group 2. Group 6 was the control group. The number of total leukocytes and cell classification in bronchoalveolar lavage (BALF) were counted, and inflammatory characteristic of lung was scored by staining with hematoxylin and eosin. The protein expressions of transforming growth factor (TGF-β1), interleukin-6 (IL-6), interleukin-17 (IL-17A) in lung of the mice were detected by immunohistochemical method. The activation of neutrophils in lung was assayed by the level of myeloroxidase (MPO).
There was no inflammatory cells infiltration in lung of the mice in group 1. Compared with group 6, numbers of total leukocytes and erythrocytes as well as the percentage of neutrophils and lymphocytes were increased in group 2. Inflammatory score and protein expressions of TGF-β1 [(75 437 ± 3 638) vs. (6 118 ± 1 978)], IL-6 [(121 650 ± 25 389) vs. (15 726 ± 9 360)], IL-17A [(252 105 ± 31 651)vs. (72 644 ± 12 285)] in lung were increased, too. Inflammatory score and TGF-β1 (11 054 ± 1 468), IL-6 (50 877 ± 11 744), IL-17A (137 864 ± 28 986) expressions in group 5 were lower than those in group 2. Eosinophils infiltration was significant in group 5. After the treatment with Ful, TGF-β1 expression did not change and IL-6, IL-17A expressions were decreased in lung of the mice that inhaled OVA. It was not enough for Ful to relieve the neutrophil aggregation and improve inflammatory reaction in lung.
The expressions of TGF-β1, IL-6, IL-17A in lung of the mice with OVA allergy were increased markedly after they inhaled specific antigen, which caused serious inflammation that was induced by neutrophil infiltration in lung. Ful could decrease the expressions of IL-6, IL-17A to some extent, but it was not enough to improve pathologic state in lung.
探讨卵清蛋白（OVA）致敏的BALB/c小鼠肺部免疫状态及黄褐毛忍冬苷（Ful）对小鼠肺部的影响，为食物过敏患者易患哮喘的机制及中药治疗效果观察提供线索。
96只雌性BALB/c小鼠随机分为6组。第1组和第2组小鼠经腹腔注射致敏、经口灌胃激发OVA，分别通过雾化吸入暴露于磷酸盐缓冲液和OVA。第3组和第4组小鼠用Ful治疗，其他过程分别与第1组和第2组小鼠相同。第5组小鼠未经口灌胃激发OVA，其他过程与第2组小鼠相同。第6组为对照组。计数支气管肺泡灌洗（BALF）中白细胞总数及细胞分类，用苏木精-伊红染色对肺部炎症特征进行评分。采用免疫组化法检测小鼠肺部转化生长因子（TGF-β1）、白细胞介素-6（IL-6）、白细胞介素-17（IL-17A）的蛋白表达。通过髓过氧化物酶（MPO）水平检测肺部中性粒细胞的活化情况。
第1组小鼠肺部无炎症细胞浸润。与第6组相比，第2组小鼠白细胞总数、红细胞数以及中性粒细胞和淋巴细胞百分比均增加。肺部炎症评分及TGF-β1[（75437±3638）对（6118±1978）]、IL-6[（121650±25389）对（15726±9360）]、IL-17A[（252105±31651）对（72644±12285）]的蛋白表达也增加。第5组肺部炎症评分及TGF-β1（11054±1468）、IL-6（50877±11744）、IL-17A（137864±28986）表达低于第2组。第5组嗜酸性粒细胞浸润明显。Ful治疗后，吸入OVA的小鼠肺部TGF-β1表达未改变，IL-6、IL-17A表达降低。Ful不足以缓解肺部中性粒细胞聚集及改善炎症反应。
OVA致敏小鼠吸入特异性抗原后，肺部TGF-β1、IL-6、IL-17A表达明显增加，导致肺部中性粒细胞浸润引起的严重炎症。Ful可在一定程度上降低IL-6、IL-17A表达，但不足以改善肺部病理状态。
