Yu Fu-sheng, Zhang Yan, Feng Yan, Zhang Ling, Ma Yan-hong, Song Wei, Hou Yue-mei
Laboratory of Arrhythmia, Frist Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2010 Jul;38(7):644-7.
To evaluate the nerve remodeling induced by 48 hours right atrial pacing in a canine model.
Rapid right atrial pacing (600 beats/min) was performed in 6 mongrel dogs of either sex for 48 hours to induce sustained atrial fibrillation (AF). Six dogs without pacing served as controls. Cardiac nerves were immunocytochemically stained using anti-growth-associated protein 43 (GAP43) and anti-choline acetyltransferase (CHAT) antibodies to compare nerve sprouting and pneumogastric nerve remodeling between the 2 groups.
In dogs with AF, the GAP43-positive and CHAT-positive nerve densities in the left atrium, left auricular appendage, right atrium and right auricular appendage were significantly higher than in control animals (all P < 0.05). Moreover, nerve density was significantly higher in the right atrium than in the left atrium in dogs with AF. Microscopic examinations revealed an inhomogeneous distribution of cardiac nerves.
Significant nerve sprouting and pneumogastric nerve remodeling were evidenced in the right and left atrium in a canine model of sustained AF induced by 48 hours right atrial pacing.
评估犬模型中48小时右心房起搏诱导的神经重塑。
对6只不同性别的杂种犬进行快速右心房起搏（600次/分钟），持续48小时以诱导持续性心房颤动（AF）。6只未起搏的犬作为对照。使用抗生长相关蛋白43（GAP43）和抗胆碱乙酰转移酶（CHAT）抗体对心脏神经进行免疫细胞化学染色，以比较两组之间的神经发芽和迷走神经重塑。
在患有AF的犬中，左心房、左心耳、右心房和右心耳中GAP43阳性和CHAT阳性神经密度显著高于对照动物（所有P<0.05）。此外，患有AF的犬右心房神经密度显著高于左心房。显微镜检查显示心脏神经分布不均匀。
在48小时右心房起搏诱导的持续性AF犬模型中，左右心房均出现明显的神经发芽和迷走神经重塑。
