[变应性鼻炎患者外周血血清氧化应激变化]

[Changes of oxidative stress in peripheral serum of patients with allergic rhinitis].

作者信息

Jiao Jian, Zhang Wei, Meng Na, Wang Hong, Zhang Luo

机构信息

Beijing Institute of Otorhinolaryngology, Key Laboratory of Otorhinolaryngology, Head and Neck Surgery (Capital Medical University), Ministry of Education, Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Affilitiated to Capital Medical University, Beijing 100005, China.

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Jun;45(6):455-8.

PMID:21055321

Abstract

OBJECTIVE

To examine the changes of oxidative stress biomarkers in peripheral serum of patients with allergic rhinitis (AR), so as to investigate the role of oxidative stress in pathogenesis of AR.

METHODS

The levels of serum nitric oxide (NO), malondialdehyde (MDA), as well as the activities of serum nitric oxide synthase (NOS), antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured in 48 AR patients and 23 healthy controls. Statistical analysis was performed using a SPSS 13.0 software.

RESULTS

The level of serum NO in AR group [(97.92 ± 73.42) µmol/L] was higher than that in healthy control group [(64.04 ± 29.54) µmol/L], the difference was significant (t = -0.281, P < 0.05). The ratio of inducible NOS (iNOS) to total NOS (TNOS) in AR group (0.51 ± 0.11) was higher than that in healthy control group(0.45 ± 0.15), the difference was significant (t = -2.061, P < 0.05). The activity of serum GSH-Px in AR group [(258.24 ± 45.25) U/(ml·min)] was higher than that in healthy control group (215.11 ± 47.62) U/(ml·min)], the difference was significant (t = -2.235, P < 0.05). Although activity of SOD, concentration of MDA in AR group had a tendency to increase and decrease, respectively, no significant statistical difference was found as compared to the control group (P > 0.05).

CONCLUSION

Oxidative stress is involved in the pathophysiology of AR, however, iNOS-NO pathway may play a more important role in AR pathogenesis.

摘要

目的

检测变应性鼻炎（AR）患者外周血血清中氧化应激生物标志物的变化，探讨氧化应激在AR发病机制中的作用。

方法

检测48例AR患者和23名健康对照者血清一氧化氮（NO）、丙二醛（MDA）水平，以及血清一氧化氮合酶（NOS）、抗氧化酶超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。采用SPSS 13.0软件进行统计学分析。

结果

AR组血清NO水平为（97.92±73.42）μmol/L，高于健康对照组的（64.04±29.54）μmol/L，差异有统计学意义（t=-0.281，P<0.05）。AR组诱导型NOS（iNOS）与总NOS（TNOS）的比值为0.51±0.11，高于健康对照组的0.45±0.15，差异有统计学意义（t=-2.061，P<0.05）。AR组血清GSH-Px活性为（258.24±45.25）U/（ml·min），高于健康对照组的（215.11±47.62）U/（ml·min），差异有统计学意义（t=-2.235，P<0.05）。AR组SOD活性、MDA浓度虽分别有升高和降低趋势，但与对照组比较差异无统计学意义（P>0.05）。