Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
J Proteomics. 2011 Feb 1;74(2):212-30. doi: 10.1016/j.jprot.2010.10.008. Epub 2010 Nov 3.
In potato (Solanum tuberosum), a signal is delivered from the leaf to underground organs to activate tuber formation. Short-day (SD) conditions induce tuberization and long-day (LD) inhibits the process. Recent studies have implicated a mobile RNA, StBEL5, as a potential signal in this process. The petiole constitutes an important vascular channel for the transport of light-mediated signals originating from the leaf blade and is also the transcriptional source of StBEL5 RNA. Hence, identifying the proteins in the petiole and their differential expression under SD and LD photoperiods will be helpful in further understanding the downstream signaling process. Thus, we have undertaken a proteomic analysis of proteins isolated from potato petioles (PP) grown under LD and SD photoperiod conditions using 2-dimensional gel electrophoresis (2-DE) followed by mass spectrometry based identification of proteins (a total of 125 proteins were identified from 185 spots). Sixty-seven differentially expressed proteins were identified in response to SD or LD photoperiods and an additional 43 putative phosphoproteins were identified through affinity enrichment. Numerous poly(U)-binding proteins which contain RNA recognition motifs have also been isolated and identified. This is the first comprehensive proteomics study that examines and catalogs proteins present in the potato petiole.
在马铃薯（Solanum tuberosum）中，一个信号从叶片传递到地下器官，激活块茎形成。短日照（SD）条件诱导块茎形成，长日照（LD）抑制该过程。最近的研究表明，一种移动 RNA，StBEL5，可能是该过程中的潜在信号。叶柄构成了从叶片起源的光介导信号的重要血管通道，也是 StBEL5 RNA 的转录源。因此，鉴定叶柄中的蛋白质及其在 SD 和 LD 光周期下的差异表达将有助于进一步了解下游信号转导过程。因此，我们使用二维凝胶电泳（2-DE）对在 LD 和 SD 光周期条件下生长的马铃薯叶柄（PP）中的蛋白质进行了蛋白质组学分析，随后通过基于质谱的蛋白质鉴定（从 185 个斑点中鉴定了总共 125 种蛋白质）。响应 SD 或 LD 光周期，鉴定出 67 种差异表达的蛋白质，并通过亲和富集鉴定出另外 43 种假定磷酸化蛋白质。还分离和鉴定了许多含有 RNA 识别基序的多聚（U）结合蛋白。这是第一个全面的蛋白质组学研究，检查和编目了存在于马铃薯叶柄中的蛋白质。
