Lehesranta Satu J, Davies Howard V, Shepherd Louise V T, Koistinen Kaisa M, Massat Nathalie, Nunan Naoise, McNicol James W, Kärenlampi Sirpa O
Institute of Applied Biotechnology, University of Kuopio, Kuopio, Finland.
Proteomics. 2006 Nov;6(22):6042-52. doi: 10.1002/pmic.200600383.
The tuber of potato (Solanum tuberosum) is commonly used as a model for underground storage organs. In this study, changes in the proteome were followed from tuberization, through tuber development and storage into the sprouting phase. Data interrogation using principal component analysis was able to clearly discriminate between the various stages of the tuber life cycle. Moreover, five well-defined protein expression patterns were found by hierarchical clustering. Altogether 150 proteins showing highly significant differences in abundance between specific stages in the life cycle were highlighted; 59 of these were identified. In addition, 50 proteins with smaller changes in abundance were identified, including several novel proteins. Most noticeably, the development process was characterized by the accumulation of the major storage protein patatin isoforms and enzymes involved in disease and defense reactions. Furthermore, enzymes involved in carbohydrate and energy metabolism and protein processing were associated with development but decreased during tuber maturation. These results represent the first comprehensive picture of many proteins involved in the tuber development and physiology.
马铃薯（Solanum tuberosum）块茎通常被用作地下贮藏器官的模型。在本研究中，从块茎形成开始，跟踪蛋白质组的变化，历经块茎发育、贮藏，直至发芽阶段。使用主成分分析进行数据查询能够清晰地区分块茎生命周期的各个阶段。此外，通过层次聚类发现了五种明确的蛋白质表达模式。总共突出显示了150种在生命周期特定阶段丰度存在高度显著差异的蛋白质；其中59种被鉴定出来。此外，还鉴定出50种丰度变化较小的蛋白质，包括几种新蛋白质。最值得注意的是，发育过程的特征是主要贮藏蛋白马铃薯Patatin同工型以及参与疾病和防御反应的酶的积累。此外，参与碳水化合物和能量代谢以及蛋白质加工的酶与发育相关，但在块茎成熟过程中减少。这些结果代表了参与块茎发育和生理学的众多蛋白质的首张综合图谱。
