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通过描述性感官分析、气相色谱嗅闻和偏最小二乘回归评估牛肉基料对牛肉味加工香精香气特征的贡献。

Contribution of beef base to aroma characteristics of beeflike process flavour assessed by descriptive sensory analysis and gas chromatography olfactometry and partial least squares regression.

机构信息

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214112 Jiangsu, China.

出版信息

J Chromatogr A. 2010 Dec 3;1217(49):7788-99. doi: 10.1016/j.chroma.2010.10.046. Epub 2010 Oct 20.

Abstract

Descriptive sensory analysis and gas chromatography-mass spectrometry (GC-MS) analysis were conducted to investigate changes in aroma characteristics of beeflike process flavours (BPFs) prepared from enzymatically hydrolyzed beef (beef base) of different DH (degree of hydrolysis) with other ingredients. Five attributes (beefy, meaty, simulate, mouthful and roasted) were selected to assess BPFs. The results of descriptive sensory analysis confirmed that BPF2 from beef base of moderate DH 29.13% was strongest in beefy, meaty and simulate characteristics; BPF4 and BPF5 from beef base of higher DH (40.43% and 44.22%, respectively) were superior in mouthful and roasted attributes respectively; while BPF0 without beef base gave weaker odour for all attributes. Twenty six compounds from GC-MS were selected as specific compounds to represent beef odour based on their odour-active properties assessed by a detection frequency method of GC-O and correlation of their contents with sensory attributes intensity. Correlation analysis of molecular weight (MW) of peptides, odour-active compounds and sensory attributes through partial least squares regression (PLSR) further explained that beef base with DH of 29.13% was a desirable precursor for imparting aroma characteristics of beeflike process flavour.

摘要

采用描述性感官分析和气相色谱-质谱联用(GC-MS)分析方法,研究了不同水解度(DH)的酶解牛肉(牛肉基)与其他成分制备的牛肉味香精(BPF)的香气特征变化。选择了 5 种属性(牛肉味、肉味、模拟味、口感和烤味)来评估 BPF。描述性感官分析的结果证实,DH 为 29.13%的适度水解牛肉基的 BPF2 在牛肉味、肉味和模拟味方面最强;DH 较高(分别为 40.43%和 44.22%)的 BPF4 和 BPF5 在口感和烤味方面表现优异;而没有牛肉基的 BPF0 在所有属性上的气味都较弱。根据 GC-O 检测频率法评估的气味活性特性,从 GC-MS 中选择了 26 种化合物作为代表牛肉气味的特定化合物,并与感官属性强度相关联。通过偏最小二乘回归(PLSR)对肽、气味活性化合物和感官属性的分子量(MW)进行相关性分析,进一步解释了 DH 为 29.13%的牛肉基是赋予牛肉味香精香气特征的理想前体。

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