Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, 20131 Milan, Italy.
J Proteomics. 2011 Jan 1;74(1):127-36. doi: 10.1016/j.jprot.2010.10.009. Epub 2010 Nov 5.
The cytoplasmic proteome of spinach leaves (Spinacia oleracea L) has been investigated with the help of commercially available (ProteoMiner) combinatorial peptide ligand libraries and with home-made ligand beads as prepared in our laboratory. The protein capture had been performed at three pH values (4.0, 7.0 and 9.3) and elution performed in 4% boiling SDS, 20mM DTT. The total number of unique gene products identified amounts to 322 proteins, of which 114 are in common with the control, untreated sample, 18 are present only in the control and 190 represent the new species detected with the help of all combined eluates and likely represent low-abundance species. This is the first in depth exploration of the spinach cytoplasmic proteome and might enable further studies on interaction, regulation and expression of proteins biological processes in combination or not with transcriptomics data.
利用市售的（ProteoMiner）组合肽配体文库和我们实验室自制的配体珠，研究了菠菜叶片（Spinacia oleracea L）的细胞质蛋白质组。在三个 pH 值（4.0、7.0 和 9.3）下进行蛋白质捕获，并在 4%沸腾 SDS、20mM DTT 中洗脱。鉴定的独特基因产物总数达到 322 种蛋白质，其中 114 种与对照、未处理的样品共有，18 种仅存在于对照中，190 种代表用所有组合洗脱液检测到的新物种，可能代表低丰度物种。这是对菠菜细胞质蛋白质组的首次深入探索，可能使进一步研究蛋白质生物过程的相互作用、调节和表达成为可能，而无需结合转录组学数据。
