Department of Analytical Chemistry, University of Alcalá, Madrid, Spain.
Electrophoresis. 2012 Sep;33(18):2799-805. doi: 10.1002/elps.201200254.
Avocado (Persea americana) proteins have been scarcely studied despite their importance, especially in food related allergies. The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries at pH 7.4 and under conditions mimicking reverse-phase capture at pH 2.2. The total number of unique gene products identified amounts to 1012 proteins, of which 174 are in common with the control, untreated sample, 190 are present only in the control and 648 represent the new species detected via combinatorial peptide ligand libraries of all combined eluates and likely represent low-abundance proteins. Among the 1012 proteins, it was possible to identify the already known avocado allergen Pers a 1 and different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin-like protein, a glucanase, and an isoflavone reductase like protein.
鳄梨（Persea americana）蛋白虽然很重要，尤其是在与食物相关的过敏方面，但却很少被研究。本研究通过在 pH 7.4 下用组合肽配体文库提取蛋白质，并在 pH 2.2 下模拟反相捕获条件，深入研究了鳄梨果肉的蛋白质组。鉴定出的独特基因产物总数达到 1012 种蛋白质，其中 174 种与对照未处理样品共有，190 种仅存在于对照样品中，648 种代表通过组合肽配体文库检测到的新物种，这些新物种可能是所有组合洗脱液中的低丰度蛋白质。在这 1012 种蛋白质中，可以鉴定出已有的鳄梨过敏原 Pers a 1 和其他可能成为过敏原的蛋白质，如丝氨酸蛋白酶抑制剂、多聚半乳糖醛酸酶、类硫氧还蛋白、葡聚糖酶和异黄酮还原酶样蛋白。
