Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, 510515 Guangzhou, China.
Med Oncol. 2011 Dec;28 Suppl 1:S341-8. doi: 10.1007/s12032-010-9727-5. Epub 2010 Nov 5.
To scan differentially expressed genes and to identify candidate molecular markers in nasopharyngeal carcinoma (NPC), we analyzed cDNA microarray data by GenMAPP to find specifically expressed genes in NPC and used tissue microarray and in situ hybridization techniques to confirm our microarray results. Our cDNA microarray results showed that TSPAN-1 and DPP10 genes were down-expressed in NPC, and COX7B and RFC2 genes were over-expressed in NPC. Real-time quantitative reverse transcription-PCR and in situ hybridization (ISH) techniques confirmed that TSPAN-1 and DPP10 genes had only 40.72 and 40.70% positive expression in NPC, but had high positive expression in chronic inflammation of nasopharyngeal mucosa (P < 0.01). However, COX7B and RFC2 genes were high positive rate in NPC (84.24 and 64.53%, respectively) than in normal control tissues. The data suggested that TSPAN-1, DPP10, COX7B and RFC2 genes might be the putative molecular markers of NPC.
为了扫描差异表达基因并鉴定鼻咽癌（NPC）中的候选分子标志物，我们通过 GenMAPP 分析 cDNA 微阵列数据，以找到 NPC 中特异性表达的基因，并使用组织微阵列和原位杂交技术来验证我们的微阵列结果。我们的 cDNA 微阵列结果表明，TSPAN-1 和 DPP10 基因在 NPC 中表达下调，COX7B 和 RFC2 基因在 NPC 中表达上调。实时定量逆转录-PCR 和原位杂交（ISH）技术证实，TSPAN-1 和 DPP10 基因在 NPC 中的阳性表达率仅为 40.72%和 40.70%，但在鼻咽黏膜慢性炎症中呈高阳性表达（P<0.01）。然而，COX7B 和 RFC2 基因在 NPC 中的高阳性率分别为 84.24%和 64.53%，高于正常对照组织。数据表明，TSPAN-1、DPP10、COX7B 和 RFC2 基因可能是 NPC 的潜在分子标志物。
