Sikka H C, Rutkowski J P, Kandaswami C, Kumar S, Earley K, Gupta R C
Great Lakes Laboratory, State University of New York College of Buffalo 14222.
Cancer Lett. 1990 Jan;49(1):81-7. doi: 10.1016/0304-3835(90)90142-k.
The formation and persistence of benzo[a]pyrene (BP)-DNA adducts in the liver of brown bullheads (Ictalurus nebulosus) treated with the hydrocarbon (20 mg/kg body wt, i.p.) was investigated using the 32P-postlabeling assay. The highest level of covalent binding of BP to liver DNA (188 fmol BP adducts/mg DNA) was observed 25-30 days following treatment. After 70 days, the adduct level in liver DNA had declined to approximately 26% of the maximum adduct level. One major BP-DNA adduct and several minor ones were detected in the liver. The major adduct co-chromatographed with anti-BP-7,8-diol-9,10-epoxide-deoxyguanosine (anti-BPDE-dGuo) adduct. The data suggest that brown bullheads metabolically activate BP by the same mechanism as the mammalian systems susceptible to carcinogenic effects of the hydrocarbon.
采用³²P后标记分析法，研究了用碳氢化合物（20毫克/千克体重，腹腔注射）处理的褐首鲶（美洲鲶）肝脏中苯并[a]芘（BP）-DNA加合物的形成和持久性。处理后25 - 30天观察到BP与肝脏DNA的共价结合水平最高（188飞摩尔BP加合物/毫克DNA）。70天后，肝脏DNA中的加合物水平已降至最大加合物水平的约26%。在肝脏中检测到一种主要的BP-DNA加合物和几种次要的加合物。主要加合物与抗BP-7,8-二醇-9,10-环氧化物-脱氧鸟苷（抗BPDE-dGuo）加合物共色谱。数据表明，褐首鲶通过与易受该碳氢化合物致癌作用影响的哺乳动物系统相同的机制代谢激活BP。
