Mann R S, Hogness D S
Department of Biochemistry, Beckman Center, Stanford University School of Medicine, California 94305.
Cell. 1990 Feb 23;60(4):597-610. doi: 10.1016/0092-8674(90)90663-y.
Expression of Ultrabithorax (UBX) proteins via a heat-inducible promoter generated homeotic transformations of segmental identities in the embryonic cuticle and peripheral nervous system (PNS) of Drosophila and transformed antennae into legs in the adult. The embryonic transformations were used to determine the identity functions of members of the UBX family and UBX mutant forms. Whereas UBX forms I and IV each induced the cuticle transformations, only form I induced the PNS transformations. Analysis of the transformations generated by UBX deletions and by a chimeric Ultrabithorax-Antennapedia protein demonstrated that the majority of the UBX identity information is contained within the C-terminal, homeodomain-containing portion of the protein. Implications of these results for how homeotic proteins select particular metameric identities are discussed.
通过热诱导启动子表达超双胸（UBX）蛋白，在果蝇的胚胎表皮和外周神经系统（PNS）中产生了体节身份的同源异型转化，并在成虫中将触角转化为腿。胚胎转化被用于确定UBX家族成员和UBX突变形式的身份功能。虽然UBX形式I和IV都诱导了表皮转化，但只有形式I诱导了PNS转化。对由UBX缺失和嵌合的超双胸-触角足蛋白产生的转化分析表明，大部分UBX身份信息包含在该蛋白含同源结构域的C末端部分。讨论了这些结果对同源异型蛋白如何选择特定体节身份的意义。
