运动引起的马的肌肉和血液中炎症细胞因子增加。

Exercise-induced increases in inflammatory cytokines in muscle and blood of horses.

作者信息

Liburt N R, Adams A A, Betancourt A, Horohov D W, McKeever K H

机构信息

Department of Animal Sciences, Equine Science Center, Rutgers State University of New Jersey, USA.

出版信息

Equine Vet J Suppl. 2010 Nov(38):280-8. doi: 10.1111/j.2042-3306.2010.00275.x.

DOI:10.1111/j.2042-3306.2010.00275.x

PMID:21059019

Abstract

REASONS FOR PERFORMING STUDY

Studies have demonstrated increases in mRNA expression for inflammatory cytokines following exercise in horses and have suggested those markers of inflammation may play a role in delayed onset muscle soreness. However, measurement of mRNA expression in white blood cells is an indirect method. No studies to date have documented the cytokine response to exercise directly in muscle in horses.

HYPOTHESIS

This study tested the hypothesis that exercise increases cytokine markers of inflammation in blood and muscle.

METHODS

Blood and muscle biopsies were obtained from 4 healthy, unfit Standardbred mares (∼ 500 kg). The randomised crossover experiment was performed with the investigators performing the analysis blind to the treatment. Each horse underwent either incremental exercise test (GXT) or standing parallel control with the trials performed one month apart. During the GXT horses ran on a treadmill (1 m/s increases each min until fatigue, 6% grade). Blood and muscle biopsies were obtained 30 min before exercise, immediately after exercise and at 0.5, 1, 2, 6 and 24 h post GXT or at matched time points during the parallel control trials. Samples were analysed using real time-PCR for measurement of mRNA expression of interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 (IL-1). Data were analysed using t tests with the null hypothesis rejected when P < 0.10.

RESULTS

There were no changes (P > 0.10) in IL-1, IL-6, IFN-gamma or TNF-alpha during control. Exercise induced significant increases in IFN-gamma, IL1 and TNF-alpha in blood and significant increases in IFN-gamma, IL-6 and TNF-alpha in muscle. There were no significant changes in mRNA expression of IL-1 in muscle or IL-6 in blood following the GXT. These cytokine markers of inflammation all returned to preGXT levels by 24 h post GXT.

CONCLUSION

High intensity exercise results in a transient increase in the expression of inflammatory cytokines in muscle and blood.

摘要

开展本研究的原因

研究表明，马匹运动后炎性细胞因子的mRNA表达会增加，并且提示这些炎症标志物可能在延迟性肌肉酸痛中起作用。然而，测量白细胞中的mRNA表达是一种间接方法。迄今为止，尚无研究记录马匹肌肉对运动的细胞因子反应。

假设

本研究检验了运动可增加血液和肌肉中炎症细胞因子标志物这一假设。

方法

从4匹健康、不健壮的标准赛马母马（约500千克）身上采集血液和肌肉活检样本。进行随机交叉实验，分析人员在对治疗不知情的情况下进行分析。每匹马接受递增运动试验（GXT）或站立平行对照，试验间隔1个月进行。在GXT期间，马匹在跑步机上跑步（每分钟速度增加1米/秒直至疲劳，坡度为6%）。在运动前30分钟、运动后立即以及GXT后0.5、1、2、6和24小时或平行对照试验的匹配时间点采集血液和肌肉活检样本。使用实时PCR分析样本，以测量干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1（IL-1）的mRNA表达。使用t检验分析数据，当P<0.10时拒绝原假设。

结果

在对照期间，IL-1、IL-6、IFN-γ或TNF-α无变化（P>0.10）。运动导致血液中IFN-γ、IL-1和TNF-α显著增加，肌肉中IFN-γ、IL-6和TNF-α显著增加。GXT后，肌肉中IL-1或血液中IL-6的mRNA表达无显著变化。这些炎症细胞因子标志物在GXT后24小时均恢复到GXT前水平。