Département de Sciences Cliniques, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
J Vet Intern Med. 2011 Mar-Apr;25(2):322-9. doi: 10.1111/j.1939-1676.2010.0664.x. Epub 2011 Jan 31.
There is limited information relating bronchoalveolar lavage (BAL) cytology and cytokine messenger ribonucleic acid (mRNA) expression in racehorses with inflammatory airway disease (IAD). HYPOTHESIS AND OBJECTIVE: We hypothesize that cytokine expression in BAL cells would correlate with cytology. Thus, we evaluated the mRNA expression of selected cytokines in BAL cells in racehorses with exercise intolerance and lower airway inflammation.
Thirty-one client-owned Standardbred racehorses with exercise intolerance.
Prospective, observational study. Cells were obtained by BAL, and mRNA expression of interleukin (IL)-1β, IL-4, IL-8, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ was determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR).
Nine horses had normal BAL cell differential cytology (Controls), while 22 horses had evidence of IAD based on BAL fluid cytology. Relative expressions of TNF-α/glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 0.0092 ± 0.010 versus 0.0045 ± 0.005, P= .034), IL-4/GAPDH (0.001 ± 0.002 versus 0.0003 ± 0.0003, P= .029), and IFN-γ/GAPDH (0.0027 ± 0.003 versus 0.0009 ± 0.001, P= .028) were greater in horses with IAD compared with controls. Furthermore, IL-4/GAPDH (0.001 ± 0.002 versus 0.0002 ± 0.0003, P < .0001) and IFN-γ/GAPDH (0.003 ± 0.003 versus 0.001 ± 0.001, P= .002) mRNA expression was increased in horses with increased metachromatic cell counts compared with horses with normal metachromatic cell counts. Only the mRNA expression of IL-1β/GAPDH (1.1 ± 0.7 versus 0.3 ± 0.3, P= .045) was increased with airway neutrophilia.
Differences in gene expression were associated with the presence of IAD and with specific cell types present in airway secretions of Standardbred racehorses with poor performance. These findings suggest that different pathophysiological pathways are implicated in IAD.
关于患有炎性气道疾病（IAD）的赛马的支气管肺泡灌洗（BAL）细胞学和细胞因子信使核糖核酸（mRNA）表达，相关信息有限。
我们假设 BAL 细胞中的细胞因子表达与细胞学相关。因此，我们评估了运动不耐受和下气道炎症的赛马 BAL 细胞中选定细胞因子的 mRNA 表达。
31 匹患有运动不耐受的客户所有的标准bred 赛马。
前瞻性观察性研究。通过 BAL 获得细胞，通过逆转录定量聚合酶链反应（RT-qPCR）测定白细胞介素（IL）-1β、IL-4、IL-8、肿瘤坏死因子（TNF）-α和干扰素（IFN）-γ的 mRNA 表达。
9 匹马的 BAL 细胞差异细胞学正常（对照组），而 22 匹马的 BAL 液细胞学显示存在 IAD。TNF-α/甘油醛 3-磷酸脱氢酶（GAPDH；0.0092 ± 0.010 与 0.0045 ± 0.005，P=.034）、IL-4/GAPDH（0.001 ± 0.002 与 0.0003 ± 0.0003，P=.029）和 IFN-γ/GAPDH（0.0027 ± 0.003 与 0.0009 ± 0.001，P=.028）的相对表达在患有 IAD 的马中高于对照组。此外，与正常变色细胞计数的马相比，IL-4/GAPDH（0.001 ± 0.002 与 0.0002 ± 0.0003，P <.0001）和 IFN-γ/GAPDH（0.003 ± 0.003 与 0.001 ± 0.001，P=.002）的 mRNA 表达在变色细胞计数增加的马中增加。只有 IL-1β/GAPDH（1.1 ± 0.7 与 0.3 ± 0.3，P=.045）的 mRNA 表达随着气道中性粒细胞增多而增加。
基因表达的差异与 IAD 的存在以及运动表现不佳的标准bred 赛马气道分泌物中存在的特定细胞类型有关。这些发现表明，不同的病理生理途径与 IAD 有关。
