Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, Heilongjiang, People's Republic of China.
Br J Nutr. 2011 Mar;105(6):879-86. doi: 10.1017/S0007114510004381. Epub 2010 Nov 9.
Gut hormones play key roles in the regulation of energy homeostasis. However, little is known about the long- and short-term effects of changing dietary fat content on gut hormones. We aim to examine the effects of changing dietary fat content on plasma gut hormone concentrations in diet-induced obese (DIO) and diet-resistant (DR) rats. After inducing obesity with a high-fat (HF) diet, male Sprague-Dawley rats were divided into three groups according to their body-weight gain: DIO; DR; control (CON). The DIO and DR rats were further divided in random into two groups. One continued on a HF diet and the other switched to a low-fat (LF) diet for an additional 4 weeks. Finally, each group was randomly divided into three subgroups (n 8): fasted; fasted-refed HF; fasted-refed LF diet groups. Replacing a HF diet with a LF diet for 4 weeks resulted in less fat mass, higher fasting and post-HF plasma ghrelin concentration and lower postprandial plasma cholecystokinin concentration in the DIO and DR rats. Acute switching dietary fat resulted in significantly higher post-HF plasma ghrelin concentrations than post-LF ghrelin concentrations in the DR rats on LF diet (DRLF) and DIO rats on LF diet (DIOLF) rats, and significantly higher post-HF obestatin concentrations than post-LF obestatin concentrations in the CON, DR rats on HF diet (DRHF) and DRLF rats. Dietary fat content appears to play a role in the gut hormone profile, which may consequently influence fat mass.
肠道激素在能量平衡的调节中起着关键作用。然而，关于改变饮食脂肪含量对肠道激素的长期和短期影响知之甚少。我们旨在研究改变饮食脂肪含量对饮食诱导肥胖（DIO）和饮食抵抗（DR）大鼠血浆肠道激素浓度的影响。在高脂肪（HF）饮食诱导肥胖后，雄性 Sprague-Dawley 大鼠根据体重增加分为三组：DIO；DR；对照（CON）。DIO 和 DR 大鼠进一步随机分为两组。一组继续喂食 HF 饮食，另一组在额外的 4 周内切换为低脂肪（LF）饮食。最后，每组随机分为三个亚组（n 8）：禁食；禁食-再喂食 HF 饮食；禁食-再喂食 LF 饮食组。用 LF 饮食替代 HF 饮食 4 周可使 DIO 和 DR 大鼠的体脂减少，空腹和 HF 后血浆胃饥饿素浓度升高，餐后胆囊收缩素浓度降低。急性切换饮食脂肪可导致 DR 大鼠 LF 饮食（DRLF）和 DIO 大鼠 LF 饮食（DIOLF）的 HF 后血浆胃饥饿素浓度显著高于 LF 后胃饥饿素浓度，CON、DR 大鼠 HF 饮食（DRHF）和 DRLF 大鼠的 HF 后肥胖素浓度显著高于 LF 后肥胖素浓度。饮食脂肪含量似乎在肠道激素谱中起作用，这可能会影响脂肪量。
