[非小细胞肺癌中p53、细胞周期蛋白D1和增殖细胞核抗原的表达]

[The expression of p53, cyclin D1 and PCNA in non-small cell lung cancer].

作者信息

Wang X, Li Y, Zhao H, Zhao T, Zhang N, Ma Y, Yang J

机构信息

Department of Pathology , TianjinMedical University , Tianjin 300070, P. R. China.

出版信息

Zhongguo Fei Ai Za Zhi. 2001 Oct 20;4(5):321-3. doi: 10.3779/j.issn.1009-3419.2001.05.01.

DOI:10.3779/j.issn.1009-3419.2001.05.01

PMID:21059306

Abstract

BACKGROUND

To study the expression of p53, cyclin D1 proteins and their relations to cell proliferation in non-small cell lung cancer ( NSCLC) .

METHODS

The expression of p53, cyclin D1 proteins and PCNA was studied in 74 cases of NSCLC and 10 cases of normal lung tissues by SP immunohistochemical technique.

RESULTS

Positive rates of p53 and cyclin D1 protein expression in NSCLC were 55. 41% and 37. 84% respectively, which were both significantly higher than those in normal lung tissues ( P₁ = 0. 003 1, P₂= 0. 042 9) . p53 protein expression correlated remarkably with lymphatic metastasis ( P= 0. 022 2) . p53 and cyclin D1 protein expression correlated significantly with PCNA index respectively ( P₁ = 0. 001 0, P₂ = 0. 000 9) . There was no significant correlation between p53 and cyclin D1 proteins ( P > 0. 05) .

CONCLUSIONS

p53, cyclin D1 protein overexpression plays an important role in tumorigenesis and cell proliferation of NSCLC. p53 is also an important indicator in the diagnosis and prognostic evaluation of NSCLC.

摘要

背景

研究p53、细胞周期蛋白D1蛋白的表达及其与非小细胞肺癌（NSCLC）细胞增殖的关系。

方法

采用SP免疫组化技术检测74例NSCLC组织及10例正常肺组织中p53、细胞周期蛋白D1蛋白及增殖细胞核抗原（PCNA）的表达。

结果

NSCLC组织中p53和细胞周期蛋白D1蛋白的阳性表达率分别为55.41%和37.84%，均显著高于正常肺组织（P₁ = 0.003 1，P₂ = 0.042 9）。p53蛋白表达与淋巴结转移显著相关（P = 0.022 2）。p53和细胞周期蛋白D1蛋白表达分别与PCNA指数显著相关（P₁ = 0.001 0，P₂ = 0.000 9）。p53与细胞周期蛋白D1蛋白之间无显著相关性（P > 0.05）。