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GenopalTM：一种新型的中空纤维阵列，用于聚焦微阵列分析。

Genopal™: a novel hollow fibre array for focused microarray analysis.

机构信息

Department of Molecular Genetics, Research Institute for Microbial Diseases, Suita, Osaka, Japan.

出版信息

DNA Res. 2010 Dec;17(6):369-79. doi: 10.1093/dnares/dsq025. Epub 2010 Nov 8.

DOI:10.1093/dnares/dsq025
PMID:21059707
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2993541/
Abstract

Expression profiling of target genes in patient blood is a powerful tool for RNA diagnosis. Here, we describe Genopal™, a novel platform ideal for efficient focused microarray analysis. Genopal™, which consists of gel-filled fibres, is advantageous for high-quality mass production via large-scale slicing of the Genopal™ block. We prepared two arrays, infectant and autoimmunity, that provided highly reliable data in terms of repetitive scanning of the same and/or distinct microarrays. Moreover, we demonstrated that Genopal™ had sensitivity sufficient to yield signals in short hybridization times (0.5 h). Application of the autoimmunity array to blood samples allowed us to identify an expression pattern specific to Takayasu arteritis based on the Spearman rank correlation by comparing the reference profile with those of several autoimmune diseases and healthy volunteers (HVs). The comparison of these data with those obtained by other methods revealed that they exhibited similar expression profiles of many target genes. Taken together, these data demonstrate that Genopal™ is an advantageous platform for focused microarrays with regard to its low cost, rapid results and reliable quality.

摘要

患者血液中靶基因的表达谱分析是 RNA 诊断的有力工具。在这里，我们描述了 Genopal™，这是一种新型平台，非常适合高效的聚焦微阵列分析。Genopal™由充满凝胶的纤维组成，通过大规模切割 Genopal™ 块，有利于高质量的大规模生产。我们准备了两个阵列，感染和自身免疫，在相同和/或不同微阵列的重复扫描方面提供了高度可靠的数据。此外，我们证明 Genopal™ 具有足够的灵敏度，可以在短杂交时间（0.5 小时）内产生信号。将自身免疫阵列应用于血液样本，通过比较参考图谱与几种自身免疫性疾病和健康志愿者（HV）的图谱，我们可以根据 Spearman 秩相关来确定大动脉炎特有的表达模式。将这些数据与其他方法获得的数据进行比较表明，它们显示出许多靶基因的相似表达谱。总之，这些数据表明，Genopal™ 是一种具有成本效益、快速结果和可靠质量的聚焦微阵列的有利平台。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/fdc1c3ed4337/dsq02507.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/41dbd20030b0/dsq02501.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/b109679c24ea/dsq02502.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/e7bd6128f6e8/dsq02503.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/690c70d93b88/dsq02504.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/35b7eb538f79/dsq02505.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/fb2bfa68d3a2/dsq02506.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/fdc1c3ed4337/dsq02507.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/41dbd20030b0/dsq02501.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/b109679c24ea/dsq02502.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/e7bd6128f6e8/dsq02503.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/690c70d93b88/dsq02504.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/35b7eb538f79/dsq02505.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/fb2bfa68d3a2/dsq02506.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c512/2993541/fdc1c3ed4337/dsq02507.jpg

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